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pHH0103_ARHGAP10-1/1-SV1/2

Plasmid

#91286

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP10-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP10-1/1-SV1/2 (ARHGAP10 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_KALRN-4/4

Plasmid

#91289

Purpose

Protein expression and purification of human SH3 domain construct KALRN-4/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

KALRN-4/4 (KALRN Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CACNB3-1/1-SV2/2

Plasmid

#91290

Purpose

Protein expression and purification of human SH3 domain construct CACNB3-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CACNB3-1/1-SV2/2 (CACNB3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MAPK8IP2-1/1-SV2/2

Plasmid

#91293

Purpose

Protein expression and purification of human SH3 domain construct MAPK8IP2-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MAPK8IP2-1/1-SV2/2 (MAPK8IP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MYO1E-1/1-SV2/2

Plasmid

#91259

Purpose

Protein expression and purification of human SH3 domain construct MYO1E-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MYO1E-1/1-SV2/2 (MYO1E Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SRGAP2-1/1

Plasmid

#91263

Purpose

Protein expression and purification of human SH3 domain construct SRGAP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRGAP2-1/1 (SRGAP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN1-4/5

Plasmid

#91265

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-4/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-4/5 (ITSN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNMBP-1/6

Plasmid

#91276

Purpose

Protein expression and purification of human SH3 domain construct DNMBP-1/6

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DNMBP-1/6 (DNMBP Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon DREADD Gi-mCherry

Plasmid

#177669

Purpose

Expresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cells

Depositor

Karl Deisseroth

Article

Knoedler et al Cell. 2022 Feb 17;185(4):654-671.e22. do

Insert

DREADD Gi-mCherry

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Tags

mCherry

Expression

Mammalian

Promoter

nEF

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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rAAV2-retro helper

Plasmid

#81070

Purpose

retrograde AAV helper vector, carrying rep and modified capsid gene

Depositor

Alla Karpova, David Schaffer

Article

Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

Inserts

rep
rAAV2-retro cap

Use

AAV and Unspecified; Helper vector

Mutation

Pro2Ala, possibly Asp613Gly and changes made to A…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VEGFR1-V5/HIS

Plasmid

#205616

Purpose

Expression of human VEGFR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

VEGFR1 receptor tyrosine kinase (FLT1 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304-ERBB2

Plasmid

#175846

Purpose

Expresses ERBB2

Depositor

Evan Braunstein

Article

Braunstein et al Leukemia. 2016 Nov;30(11):2242-2245. doi

Insert

ERBB2 (ERBB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-mTert

Plasmid

#162555

Purpose

Overexpression of Tert

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

Overexpression of Tert (Tert Mouse)

Expression

Mammalian

Mutation

None

Promoter

EF1A

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-KIT-V5/HIS

Plasmid

#201986

Purpose

expression of human KIT receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

KIT receptor tyrosine kinase (KIT Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-HF1-plus

Plasmid

#126768

Purpose

Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1-plus

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A; Q695A; Q926A; amino acids 1005-1013 replac…

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

Plasmid

#101060

Purpose

AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IGF1R-V5/HIS

Plasmid

#201979

Purpose

expression of human IGF1R receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

IGF1R receptor tyrosine kinase (IGF1R Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RUNX2

Plasmid

#192926

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

RUNX2 (RUNX2 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-Dsup

Plasmid

#90020

Purpose

To express GFP-tagged Dsup in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PDGFRA-V5/HIS

Plasmid

#201987

Purpose

expression of human PDGFRA receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRA receptor tyrosine kinase (PDGFRA Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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