We narrowed to 11,743 results for: pcdna

1. pcDNA3.1-CAG-NanoLuc-VChR1-EYFP

   Plasmid

   #114112

   Purpose

   fusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   NanoLuc; Volvox channelrhodopsin 1; EYFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

   Plasmid

   #127308

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4-Tet-on-vsv-Incenp-EGFP

   Plasmid

   #108483

   Purpose

   inducible expression of vsv-INCENP wt-EGFP

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   INCENP (INCENP Human)
   

   Use

   Tags

   EGFP and VSV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-OTUB1 T134R,C91S-pcDNA3.1

   Plasmid

   #118212

   Purpose

   Expresses FLAG-OTUB1 T134R,C91S in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   changed cysteine 91 to serine and threonine 134 t…

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. YFP(1-158)-gamma-2 in pcDNAI/Amp

   Plasmid

   #55762

   Purpose

   An amino-terminal YFP fragment was fused to Ggamma2. When co-expressed with a carboxyl terminal YFP or CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

   Insert

   YFP(1-158)-gamma-2 (GNG2 Aequorea victoria, Human)
   

   Use

   Tags

   YFP(1-158) was fused to the amino terminus of Gga…

   Expression

   Mammalian

   Mutation

   YFP (1-158) includes a substitution of Met for Gl…

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-Fos DD T325A T331A

   Plasmid

   #8973

   Depositor

   John Blenis

   Article

   Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

   Insert

   c-Fos (Fos Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S362D, S374D, T325A, T331A

   Promoter

   Available since

   Sept. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-MAVS-M1A-invitro(KaganE12)

   Plasmid

   #52016

   Purpose

   Used for in vitro expression of the human MAVS CDS containing a point mutation M1A

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS-M1A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Met 1 to Ala

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-MAVS-G67M-invitro(KaganD100)

   Plasmid

   #52014

   Purpose

   Used for in vitro expression of the human MAVS CDS containing a point mutation G67M

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Gly 67 to Met

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-MAVS-L62M-invitro(KaganD98)

   Plasmid

   #52013

   Purpose

   Used for in vitro expression of the human MAVS CDS containing a point mutation L62M

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Leu 62 to Met

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-MAVS-E80M-invitro(KaganD99)

    Plasmid

    #52015

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation E80M

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Glu 80 to Met

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-MAVS-M142A-invitro(E13)

    Plasmid

    #52017

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation M142A

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS-M142A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Met 142 to Ala

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

12. 830 pcDNA3 GFP PTEN 1-336

    Plasmid

    #10927

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Amino acids 1-336

    Promoter

    Available since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA 3.2/V5-DEST CDC42EP1 S192A

    Plasmid

    #69820

    Purpose

    Expresses CDC42EP1-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    CDC42 effector protein (CDC42EP1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S192A

    Promoter

    CMV

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nano-lantern(Ca2+)_63/pcDNA3

    Plasmid

    #51986

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-4GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA4/HisMaxB-YAP2-1st WW mutant

    Plasmid

    #18998

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Jul 17. ():.

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Tryptophan 199 changed to Alanine, and Proline 2…

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

16. integrin alpha9 / alpha5 pcDNA1 neo

    Plasmid

    #13588

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 / alpha 5 (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-FRT-TO-vsv-Incenp wt-GFP

    Plasmid

    #108502

    Purpose

    inducible expression of vsv-INCENP wt-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP (INCENP Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

    Plasmid

    #64207

    Purpose

    mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 (CRY2 Mustard Weed)
    RGS4(del 1-33) (RGS4 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

19. XE170 mouse Wnt-1-myc-pcDNA3

    Plasmid

    #16854

    Depositor

    Randall Moon

    Insert

    Wnt1 (Wnt1 Mouse)
    

    Use

    Xenopus expression

    Tags

    myc (internal)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

20. Kv7.4 (1-645, Δ368-523)/pcDNA3.1

    Plasmid

    #111456

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523), C643A

    Promoter

    Available since

    May 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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