We narrowed to 14,023 results for: RING

1. pDONR221 CARD8 FIIND

   Plasmid

   #169963

   Purpose

   pDONR221 for Gateway shuttling. Contains the FIIND (L162M-P434, no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS406 ATG1-ATG7-3'UTR

   Plasmid

   #166847

   Purpose

   Expresses Atg1 with ATG7 3'UTR in yeasts.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

   Insert

   ATG1 (ATG1 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   ATG1 Promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS405 VAC8-G2A,C4A,C5A,C7A-RFP

   Plasmid

   #166842

   Purpose

   Expresses a myristoylation and palmitoylation mutant Vac8-RFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8 Budding Yeast)
   

   Tags

   RFP

   Expression

   Yeast

   Mutation

   G2A, C4A, C5A, C7A

   Promoter

   VAC8 promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS405 VAC8-G2A,C4A,C5A,C7A-GFP

   Plasmid

   #166839

   Purpose

   Expresses a myristoylation and palmitoylation mutant Vac8-GFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8 Budding Yeast)
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   G2A, C4A, C5A, C7A

   Promoter

   VAC8 promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS405 VAC8-C4A,C5A,C7A-GFP

   Plasmid

   #166838

   Purpose

   Expresses a palmitoylation mutant Vac8-GFP in yeasts, mutations are Cys4Ala, Cys5Ala, Cys7Ala

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8 Budding Yeast)
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   C4A, C5A, C7A

   Promoter

   VAC8 promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. IBMc180

   Plasmid

   #161942

   Purpose

   Carries Golden Gate substitution insert for acVHH-assisted bisection mapping

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
   

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBI-CMV1-mir374b~421-CMV2-mir1

   Plasmid

   #164674

   Purpose

   Express miR-374b~421 and miR-1-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-374b~421 (MIR374B Human)
   miR-1-1 (MIR1-1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBI-CMV1-mir374b-CMV2-mir1

   Plasmid

   #164672

   Purpose

   Express miR-374b and miR-1-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-374b (MIR374B Human)
   miR-1-1 (MIR1-1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBI-CMV1-mir181b-1~125-CMV2-mir1

   Plasmid

   #164671

   Purpose

   Express miR-181b-1~125a and miR-1-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-181b-1~125a (MIR181B1 Human)
   miR-1-1 (MIR1-1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. IBMc226

    Plasmid

    #161944

    Purpose

    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter
    

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRWJB006

    Plasmid

    #160113

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Promoter

    35S

    Available Since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRWJB004

    Plasmid

    #160112

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Promoter

    UBQ10

    Available Since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

    Plasmid

    #159844

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ2301 - [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159865

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-Myosin7B-sgRNA

    Plasmid

    #154861

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting Myosin7B

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    MYO7B sgRNA (MYO7B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSFDuet1-tglA(C51)HIB

    Plasmid

    #134675

    Purpose

    Expresses His6-TglA(C51), TglH, TglI and TglB in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Inserts

    TglA(C51)
    TglH
    TglI
    TglB
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    insertion of cysteine 51

    Promoter

    T7

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. TB:3xDBE_RE:MiniP

    Plasmid

    #124529

    Purpose

    3 Copies of the Daf-16 family binding element

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    3 copies of the Daf-16 family binding element
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Antares-SEPLuc IRESv24

    Plasmid

    #141087

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-SEPLuc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MT2a_K30A

    Plasmid

    #105694

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 2a K30A mutant

    Depositor

    Artur Krężel

    Article

    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

    Insert

    Metallothionein 2a (MT2A Human)
    

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Lysine 30 to Alanine

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. MT2a_K31A

    Plasmid

    #105695

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 2a K31A mutant

    Depositor

    Artur Krężel

    Article

    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

    Insert

    Metallothionein 2a (MT2A Human)
    

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Lysine 31 to Alanine

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MT2a_K30A K31A

    Plasmid

    #105696

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 2a K30/31A mutant

    Depositor

    Artur Krężel

    Article

    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

    Insert

    Metallothionein 2a (MT2A Human)
    

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Lysine 30 and 31 to Alanine

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. MT2a_C21A

    Plasmid

    #105697

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 2a C21A mutant

    Depositor

    Artur Krężel

    Article

    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

    Insert

    Metallothionein 2a (MT2A Human)
    

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Cysteine 21 to Alanine

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. MT2a_K20A

    Plasmid

    #105698

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 2a K20A mutant

    Depositor

    Artur Krężel

    Article

    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

    Insert

    Metallothionein 2a (MT2A Human)
    

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Lysine 20 to Alanine

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MT2a_S6A

    Plasmid

    #105699

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 2a S6A mutant

    Depositor

    Artur Krężel

    Article

    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

    Insert

    Metallothionein 2a (MT2A Human)
    

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Serine 6 to Alanine

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-PTEN-3'UTR

    Plasmid

    #97204

    Purpose

    Expresses PTEN 3'UTR in mammalian cells

    Depositor

    Reproducibility Project Cancer Biology

    Article

    Kerwin et al Elife. 2020 Apr 21;9. pii: 51019. doi: 1

    Insert

    PTEN 3' UTR (PTEN Human)
    

    Tags

    none

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Y365A or A365Y inducible rescue

    Plasmid

    #131323

    Purpose

    gRNA to generate i-WT-r or i-MT-r systems

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    Y365A or A365Y inducible rescue
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYPQ290-D570A-SRDX

    Plasmid

    #129682

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b-SRDX
    

    Use

    CRISPR; Gateway compatible aaccas12b entry clone

    Expression

    Plant

    Mutation

    D570A; AacCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYPQ291-D573A-SRDX

    Plasmid

    #129683

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    BthCas12b-SRDX
    

    Use

    CRISPR; Gateway compatible bthcas12b entry clone

    Expression

    Plant

    Mutation

    D573A; BthCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HoxC-downstream-5xTetO Donor

    Plasmid

    #131341

    Purpose

    Donor template to insert 5xTet operator sequence into downstream of HoxC cluster.

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    HoxC-downstream-5xTetO Donor
    

    Use

    CRISPR and Mouse Targeting

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. 5xtetO HoxC 3'

    Plasmid

    #131340

    Purpose

    gRNA to insert 5xTet operator sequence into downstream of HoxC cluster.

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    HoxC-downstream-gRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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