Addgene

We narrowed to 1,136 results for: GR

Showing: 61 - 90 of 1136 results

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pL59-Nat

Plasmid

#140465

Purpose

CRISPR/Cas9 engineering with nourseothricin selection

Depositor

Gianni Liti

Article

Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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plp

Plasmid

#22310

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Type

Empty backbone

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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plp-weakSC_GFP

Plasmid

#22311

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

GFP-rad9

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pRS403-PGAL1-strongSC_GFP

Plasmid

#22316

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

GFP-rad9-GFP

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pYES2.1-Dcr1

Plasmid

#22307

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

DCR1

Use

2micron

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pYES2.1-Ago1

Plasmid

#22306

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

AGO1

Use

2micron

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pK-11

Plasmid

#22094

Depositor

Gail Martin

Article

Meyers et al Nat Genet. 1998 Feb . 18(2):136-41.

Insert

Frt-PGKNeo-Frt-LoxP

Use

Bacterial

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pRS403-PGAL1-weakSC_GFP

Plasmid

#22315

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

GFP-rad9

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pET101-Dcr1

Plasmid

#22309

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

DCR1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only
plp-strongSC_GFP

Plasmid

#22312

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

GFP-rad9-GFP

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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LLP469 pEF1a-mCherry-EMPTY-gRNA

Plasmid

#100958

Purpose

mCherry driven by EF1a, empty gRNA scaffold driven by U6

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

gRNA empty backbone with RFP

Use

CRISPR

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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LLP453 pEf1a-NLS-aGCN4-mCherry

Plasmid

#100945

Purpose

mCherry fusion to single change variable fragment antibody GCN4 for use in SunTag system , with 3xTy1 tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

scFvGCN4, sfGFP, GB1, mCherry

Use

Tags

3xTy1

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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LLP109 pEF1a-gRNA-GFP

Plasmid

#100935

Purpose

GFP, puromycin and gRNA scaffold driven by EF1a promoter

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

gRNA scaffold

Use

Grna scaffold

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pL90-Nat-2xHO

Plasmid

#139069

Purpose

CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

Depositor

Gianni Liti

Article

Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

Insert

Two guide RNAs targeting the HO endonuclease

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

TDH3

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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LLP250 pEF1a-TALE-SunTag

Plasmid

#100939

Purpose

Empty TALE vector fused to SunTagx10 driven by EF1a promoter, with 3xHA tag, no PURO gene

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

TALE insertion site, SunTag array (10xGCN4)

Use

TALEN

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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LLP251 pEF1a-UNC5C-4-SunTag

Plasmid

#100940

Purpose

TALE vector with UNC5C-4 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

TALE target sequence for UNC5C, SunTag array (10xGCN4)

Use

TALEN

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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LLP338 pEF1a-DACH1-1-SunTag

Plasmid

#100942

Purpose

TALE vector with DACH1-1 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

TALE target sequence for DACH1, SunTag array (10xGCN4)

Use

TALEN

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRS405-PTEF-Dcr1

Plasmid

#22314

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

DCR1

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pRS404-PTEF-Ago1

Plasmid

#22313

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

AGO1

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pRS403-PGAL1-hpSC_URA3

Plasmid

#22317

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

URA hairpin

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pBJ5 mCnB

Plasmid

#17872

Depositor

Jerry Crabtree

Article

Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

Insert

CnB (Ppp3r1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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LLP339 pGK-dCas9-SunTag

Plasmid

#100943

Purpose

dCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, SunTag array (10xGCN4)

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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LLP457 pGK-dCas9-Suntag-BFP

Plasmid

#100957

Purpose

BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, SunTag array (10xGCN4)

Use

CRISPR

Tags

3xHA and BFP

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Ly6G-miR30

Plasmid

#163358

Purpose

Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker.

Depositor

Teunis Geijtenbeek, Louis Ates

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

Ly-6G (Ly6g Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-Ly6G-Rluc_miR

Plasmid

#163339

Purpose

Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker

Depositor

Teunis Geijtenbeek, Louis Ates

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

Ly-6G (Ly6g Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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SV-ABAactDA

Plasmid

#38247

Depositor

Jerry Crabtree

Article

Liang et al Sci Signal. 2011 Mar 15;4(164):rs2.

Inserts

VP16/PYL1
Gal4DBD/ABI*

Use

Tags

Flag, Gal4DBD, HA, and VP16

Expression

Mammalian

Mutation

D143A

Promoter

SV40 (IRES) and SV40p

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pBJ5 mCnA alpha

Plasmid

#17871

Depositor

Jerry Crabtree

Article

Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

Insert

CnA alpha (Ppp3ca Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pV1081

Plasmid

#111425

Purpose

Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1

Depositor

Gerald Fink

Article

Vyas et al Sci Adv. 2015;1(3):e1500248.

Insert

CaCas9/sgADE-NT2

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCHFLAGGR

Plasmid

#1279

Depositor

Susan Lindquist

Article

Kimura et al Science 1995 Jun 2;268(5215):1362-5.

Insert

GR (Nr3c1 Rat)

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
GFP-P4M-SidMx2

Plasmid

#51472

Purpose

Biosensor for PtdIns4P

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Inserts

P4M-SidM(546-647)
P4M-SidM(546-647)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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