Addgene

We narrowed to 212 results for: gak

Showing: 61 - 90 of 212 results

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pAAV-FLEX-tTA

Plasmid

#149363

Purpose

AAV-mediated and Cre-dependent expression of tTA under the CAG promoter.

Depositor

Takeshi Imai

Article

Leiwe et al bioRxiv 2022.10.20.512984

Insert

tTA2

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP

Plasmid

#48988

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Expression

Mutation

Promoter

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-8XDSR

Plasmid

#48981

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA

Plasmid

#48989

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA tag

Expression

Mutation

Promoter

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-3XDSR

Plasmid

#48976

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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LeGO-lnc

Plasmid

#80624

Purpose

Lentiviral lncRNA Overexpression

Depositor

Jan-Henning Klusmann

Article

Emmrich et al Mol Cancer. 2014 Jul 15;13:171. doi: 10.

Insert

empty control

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Cas9-EGFP

Plasmid

#159126

Purpose

Expresses active Cas9 fused with EGFP for fluorescence microscopy

Depositor

Taekjip Ha

Article

Liu et al Science. 2020 Jun 12;368(6496):1265-1269

Insert

Cas9 fused to EGFP

Use

CRISPR and Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

MSCV LTR promoter

Available since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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p3XFLAG-MKL2

Plasmid

#27175

Purpose

For mammalian expression of human MKL2 with a 3x FLAG tag

Depositor

Ron Prywes

Article

Selvaraj et al J Biol Chem. 2003 Oct 24. 278(43):41977-

Insert

MKL2 (MRTFB Human)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 22, 2010

Availability

Academic Institutions and Nonprofits only
Lyn-YFP-FRB1N

Plasmid

#162431

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Lyn-YFP-FRB1N

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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mCh-FRB1C-Cb5

Plasmid

#162432

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

mCh-FRB1C-Cb5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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mRuby-PH-PLCdelta

Plasmid

#162440

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

mRuby-PH-PLCdelta

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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Lyn-YFP-FRB1C

Plasmid

#162425

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Lyn-YFP-FRB1C

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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FRB1N-CFP-CAAX

Plasmid

#162428

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

FRB1N-CFP-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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FKBP1C-CFP-Cb5

Plasmid

#162438

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

FKBP1C-CFP-Cb5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-PM-34a-K1/2

Plasmid

#25800

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

alternative hsa-miR-34a promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
pAW8ENdeI-cHAIAA17

Plasmid

#48990

Purpose

for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

IAA17 degron tag

Expression

Mutation

Promoter

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-spo5DSR

Plasmid

#48983

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
iRFP-FRB1C-Cb5

Plasmid

#162441

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

iRFP-FRB1C-Cb5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Lyn-YFP-FKBP1N

Plasmid

#162437

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Lyn-YFP-FKBP1N

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Lyn-YFP-FRB3C

Plasmid

#162427

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Lyn-YFP-FRB3C

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FRB3N-CFP-CAAX

Plasmid

#162430

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

FRB3N-CFP-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-FRB1N-Cb5

Plasmid

#162435

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

YFP-FRB1N-Cb5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FRB2N-CFP-CAAX

Plasmid

#162429

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

FRBN-CFP-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Lyn-YFP-FRB2C

Plasmid

#162426

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Lyn-YFP-FRB2C

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CFP-FKBP

Plasmid

#162433

Purpose

for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

CFP-FKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Tom20-mCh-FKBP

Plasmid

#162436

Purpose

for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Tom20-mCh-FKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7_hsa-miR-150

Plasmid

#25792

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

hsa-miR-150 (MIR150, MYB Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pSIREN-RetroQ-MYB-shRNA

Plasmid

#25790

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

shRNA against human MYB (MYB Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pGL3-MYB-3'UTR

Plasmid

#25798

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

3'UTR of human MYB (MYB Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK2-AS34a

Plasmid

#37099

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

perfect complementary binding site for hsa-miR-34a (MIR34A Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

June 19, 2012

Availability

Academic Institutions and Nonprofits only