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Showing: 101 - 150 of 653 results

  1. pSPYngK-hp

    Plasmid
    #48118
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    signal peptide of YngK
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48118-plasmid-map-sequence-id-69944

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  2. pSPPac-hp

    Plasmid
    #48123
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    signal peptide of Pac
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48123-plasmid-map-sequence-id-69950

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  3. pSPNprM-hp

    Plasmid
    #48120
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    signal peptide of NprM
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48120-plasmid-map-sequence-id-69946

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  4. pSPVpr-hp

    Plasmid
    #48119
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    signal peptide of Vpr
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48119-plasmid-map-sequence-id-69945

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  8. pET29b(+)_gp1(A11V)

    Plasmid
    #225131
    Purpose
    Phage GIL01 protein gp1 (A11V) mutant with a single nucleotide substitution (C32T)
    Depositor
    Matej Butala
    Insert
    gp1 (A11V)
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Alanine 11 to Valine
    Promoter
    T7 promoter
    Available since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225131-plasmid-map-sequence-id-448443

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  10. pTU1-A-amyEU

    Plasmid
    #112781
    Purpose
    Upstream homology arm, amyE encodes _-amylase
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    amyEU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112781-plasmid-map-sequence-id-218371

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  11. pSPYngK+-hp

    Plasmid
    #48121
    Purpose
    Shuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    codon optimized signal peptide of YngK
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48121-plasmid-map-sequence-id-69947

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  13. pBP-P43

    Plasmid
    #112775
    Purpose
    Strong promoter activity during exponential and stationary phase
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    P43 promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112775-plasmid-map-sequence-id-218348

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  15. pBP-Pspac

    Plasmid
    #112778
    Purpose
    Promoter repressed by LacI (not natively produced in B. subtilis 168)
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    Pspac promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112778-plasmid-map-sequence-id-218361

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  16. pBP-Pveg

    Plasmid
    #112776
    Purpose
    Strong promoter active during sporulation and vegetative growth
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    Pveg promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112776-plasmid-map-sequence-id-218356

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  17. pBP-Pars

    Plasmid
    #112779
    Purpose
    Promoter of ars operon. Repressed by ArsR, induced by arsenate or arsenite
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    Pars promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112779-plasmid-map-sequence-id-218364

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  18. pTU1-D-spsCD

    Plasmid
    #112788
    Purpose
    Downstream homology arm, SpsC is involved in spore coat polysaccharide synthesis
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    spsCD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112788-plasmid-map-sequence-id-218383

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  19. pTU1-A-spsCU

    Plasmid
    #112787
    Purpose
    Upstream homology arm, SpsC is involved in spore coat polysaccharide synthesis
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    spsCU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112787-plasmid-map-sequence-id-218381

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  20. pTU1-A-recJU

    Plasmid
    #112783
    Purpose
    Upstream homology arm, RecJ is a ssDNA specifiC exonuclease, involved in genome repair and maintenance
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    recJU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112783-plasmid-map-sequence-id-218374

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  21. pTU1-C-cat

    Plasmid
    #112791
    Purpose
    Chloramphenicol resistance cassette (expressing chloramphenicol acetyltransferase)
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    cat
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112791-plasmid-map-sequence-id-218393

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  22. pTU1-D-rrnOD

    Plasmid
    #112786
    Purpose
    Downstream homology arm, rrnO-23S (23S rRNA)
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    rrnOD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112786-plasmid-map-sequence-id-218378

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  23. pTU1-D-amyED

    Plasmid
    #112782
    Purpose
    Downstream homology arm, amyE encodes _-amylase
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    amyED
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112782-plasmid-map-sequence-id-218373

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  24. pBsVchCAST-pyrE

    Plasmid
    #203813
    Purpose
    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli
    Depositor
    Sheng Yang
    Article
    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2
    Insert
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203813-plasmid-map-sequence-id-403827

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  25. pBsVchCAST

    Plasmid
    #203812
    Purpose
    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli
    Depositor
    Sheng Yang
    Article
    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2
    Insert
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203812-plasmid-map-sequence-id-403828

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  26. pBtPIPLC His

    Plasmid
    #173808
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Mary F Roberts, Anne Gershenson
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    173808-plasmid-map-sequence-id-345681

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  27. pBtPIPLC_N168C His

    Plasmid
    #173809
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Mary F Roberts, Anne Gershenson
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    Asn168Cys for labeling
    Promoter
    T7
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173809-plasmid-map-sequence-id-341161

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  28. pIA028

    Plasmid
    #200419
    Purpose
    thrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloning
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Tags
    PAmCherry1
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    200419-plasmid-map-sequence-id-405382

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  29. pIA033

    Plasmid
    #200420
    Purpose
    thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Tags
    bsPAmCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    200420-plasmid-map-sequence-id-403933

    Enlarge

  30. pIA034

    Plasmid
    #200421
    Purpose
    amyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Tags
    bsdronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200421-plasmid-map-sequence-id-405458

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  31. pBP-ORF-xylE

    Plasmid
    #112780
    Purpose
    Encodes catechol-2,3-dioxygenase, from Pseudomonas putida TOL pWW0.
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    xylE open reading frame
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112780-plasmid-map-sequence-id-218368

    Enlarge

  33. pTU1-D-recJD

    Plasmid
    #112784
    Purpose
    Downstream homology arm, RecJ is a ssDNA specific exonuclease, involved in genome repair and maintenance
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    recJD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112784-plasmid-map-sequence-id-218375

    Enlarge

  34. pTU1-A-yckABU

    Plasmid
    #112789
    Purpose
    Upstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilis
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    yckABU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112789-plasmid-map-sequence-id-218385

    Enlarge

  35. pBP-PliaG

    Plasmid
    #112777
    Purpose
    Weak constitutive promoter of liaGFSR operon involved in detection of cell wall damage
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    PliaG promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112777-plasmid-map-sequence-id-218358

    Enlarge

  37. pTU2-AmyE-Pveg-eGFP

    Plasmid
    #112792
    Purpose
    Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    Pveg-eGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112792-plasmid-map-sequence-id-218394

    Enlarge

  38. pTU1-D-yckABD

    Plasmid
    #112790
    Purpose
    Downstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilis
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    yckABD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112790-plasmid-map-sequence-id-218386

    Enlarge

  42. pIA030

    Plasmid
    #200424
    Purpose
    thrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Use
    Tags
    PAmCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Ppen
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    200424-plasmid-map-sequence-id-405349

    Enlarge

  49. pIA036

    Plasmid
    #200425
    Purpose
    amyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Use
    Tags
    bsdronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Ppen
    Available since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    200425-plasmid-map-sequence-id-443100

    Enlarge

  50. pIA035

    Plasmid
    #200426
    Purpose
    amyE::(Ppen-lacIΔ11-dronPA cm). PALM integration vector includes dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Use
    Tags
    dronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Ppen
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    200426-plasmid-map-sequence-id-405336

    Enlarge

Showing: 101 - 150 of 653 results