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Showing: 101 - 146 of 146 results

  1. Ma-Barnase

    Plasmid
    #197572
    Purpose
    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    3TAG and 45TAG
    Promoter
    araC and lpp
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197572-plasmid-map-sequence-id-388086

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  2. Ma-pREP

    Plasmid
    #197571
    Purpose
    Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    Chloramphenicol resistance gene - 111TAG
    T7 polymerase -TAG
    GFPuv
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    111TAG and 8TAG/112TAG
    Promoter
    AraC, T7, cat promoter (constitutive), and lpp (c…
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197571-plasmid-map-sequence-id-389424

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  3. pQhsCDtRNA+

    Plasmid
    #86014
    Purpose
    Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene
    Depositor
    Hong Li
    Article
    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:
    Inserts
    U14-tRNA chimera (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    Use
    Co-expression; purification
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    amino acids 1-401, amino acids 1-411, and amino a…
    Promoter
    LPP and Ptac
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86014-plasmid-map-sequence-id-169485

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  4. CMV-SEAP

    Plasmid
    #24595
    Depositor
    Alan Cochrane
    Insert
    SEAP (ALPP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 23, 2010
    Availability
    Academic Institutions and Nonprofits only
    24595-plasmid-map-sequence-id-260470

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  16. pBS-V9

    Plasmid
    #173797
    Purpose
    pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene noctiflora ClpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation
    Depositor
    Daniel Sloan
    Article
    Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.
    Inserts
    aadA
    ClpP1
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    173797-plasmid-map-sequence-id-342771

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  17. pBS-V7

    Plasmid
    #173796
    Purpose
    pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene latifolia clpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation
    Depositor
    Daniel Sloan
    Article
    Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.
    Inserts
    aadA
    ClpP1
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    173796-plasmid-map-sequence-id-342770

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  18. pBS-V6

    Plasmid
    #173795
    Purpose
    pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene conica clpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation
    Depositor
    Daniel Sloan
    Article
    Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.
    Inserts
    aadA
    ClpP1
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    173795-plasmid-map-sequence-id-342769

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  19. pBS-V4

    Plasmid
    #173794
    Purpose
    pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Nicotiana tabacum clpP1 gene with native regulatory elements and flanking regions for tobacco plastome transformation
    Depositor
    Daniel Sloan
    Article
    Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.
    Inserts
    aadA
    ClpP1
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    173794-plasmid-map-sequence-id-342763

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  20. pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)

    Plasmid
    #174516
    Purpose
    Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.
    Depositor
    Jason W Chin
    Article
    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.
    Inserts
    PylS_1R26 - CBZK
    Af_TyrRS - p-I-Phe
    Ma tRNA pylT(8) with CGA anticodon
    A.fulgidus tRNA with CUA anticodon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    CUA anticodon, M.alvus Pyl tRNA with CGA anticodo…
    Promoter
    GlnS and lpp
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    174516-plasmid-map-sequence-id-343328

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  21. XE238 hVangl2/Strabismus pCS2P+

    Plasmid
    #16992
    Depositor
    Randall Moon
    Insert
    Vangl2/Strabismus (VANGL2 Human)
    Use
    Xenopus expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 13, 2008
    Availability
    Academic Institutions and Nonprofits only

  32. pJH645

    Plasmid
    #172169
    Purpose
    Reporter plasmid for pylBCD variant in pBAD backbone
    Depositor
    Matthew Bennett
    Article
    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:
    Insert
    Pem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Inserted amber codon immediately after initiator …
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172169-plasmid-map-sequence-id-338853

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  34. pJH191

    Plasmid
    #172149
    Purpose
    Accessory Plasmid for PACE of pylBCD variants
    Depositor
    Matthew Bennett
    Article
    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:
    Insert
    Ppsp gIII.2TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    gIII(P29*, Y184*); [strong promoter eG, strong RB…
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172149-plasmid-map-sequence-id-341959

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  35. pJH61

    Plasmid
    #172143
    Purpose
    Accessory Plasmid for PACE of pylBCD variants
    Depositor
    Matthew Bennett
    Article
    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:
    Insert
    Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] pylS; PproK pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    gIII(P29*); [strong promoter eG, strong RBS SD8] …
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172143-plasmid-map-sequence-id-338567

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  36. pJH190

    Plasmid
    #172148
    Purpose
    Accessory Plasmid for PACE of pylBCD variants
    Depositor
    Matthew Bennett
    Article
    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:
    Insert
    Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    gIII(P29*); [strong promoter eG, strong RBS SD8] …
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172148-plasmid-map-sequence-id-338790

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  37. pLyGo-Ec-8

    Plasmid
    #163136
    Purpose
    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P(rhaB)
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163136-plasmid-map-sequence-id-332138

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  41. pCAGGS SARS-CoV-2 XBB Spike

    Plasmid
    #195287
    Purpose
    Expresses codon-optimized full length SARS-CoV-2 XBB Spike
    Depositor
    Marceline Côté
    Insert
    SARS-CoV-2 XBB Spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains the following mutations: T19I, LPPA24-27…
    Promoter
    chicken β-actin promoter, CMV enhancer
    Available since
    Feb. 8, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195287-plasmid-map-sequence-id-384139

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Showing: 101 - 146 of 146 results