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Showing: 101 - 130 of 130 results

  1. pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

    Plasmid
    #217014
    Purpose
    Expression of CDK2-7 chimera in E.coli
    Depositor
    Manjeet Mukherjee
    Article
    Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018
    Insert
    CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N) (CDK7, CDK2 Human)
    Use
    Tags
    C3 protease cleavable GST tag
    Expression
    Bacterial
    Mutation
    CDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)
    Promoter
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217014-plasmid-map-sequence-id-431050

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  4. TBXTA-c005

    Plasmid
    #139754
    Purpose
    For protein expression/crystallography (PDB:6F58)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-S224
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139754-plasmid-map-sequence-id-296505

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  5. TBXTA-c006

    Plasmid
    #139755
    Purpose
    For protein expression/crystallography (PDB:6F59)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-S224
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139755-plasmid-map-sequence-id-296509

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  6. RAB5AA-c006

    Plasmid
    #175476
    Purpose
    Protein expression in bacterial cells. GTPase domain, G15-N184. Can be used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    RAB5A (RAB5A Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175476-plasmid-map-sequence-id-353162

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  7. YSCD44A-c002

    Plasmid
    #175493
    Purpose
    Protein expression in bacterial cells. Single chain Fv antibody to human CD44 Link domain. Can be used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    Single chain Fv CD44 antibody (H90)
    Use
    Tags
    3XFLAG-EK-His8 and pelB
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175493-plasmid-map-sequence-id-353166

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  8. TBXTA-c021

    Plasmid
    #139758
    Purpose
    Protein expression/crystallography (PDB:5QT0)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-N211
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139758-plasmid-map-sequence-id-296541

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  9. SDCBPA-c010

    Plasmid
    #175482
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains, A106-V288. Can be used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175482-plasmid-map-sequence-id-353168

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  10. TBXTA-c020

    Plasmid
    #139757
    Purpose
    For protein expression/crystallography (PDB 5QS0)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-N211
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139757-plasmid-map-sequence-id-296518

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  11. TBXTA-c022

    Plasmid
    #139759
    Purpose
    Protein expression/crystallography
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-A214
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139759-plasmid-map-sequence-id-296542

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  12. Rab11A[8-175]-His-C

    Plasmid
    #228040
    Purpose
    Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling.
    Depositor
    Wade Harper
    Insert
    Rab11A[8-175]-His-C (RAB11A Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    228040-plasmid-map-sequence-id-450386

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  13. CD44MMA-c002

    Plasmid
    #175452
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, Q23-D172. Used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    Cd44 (Cd44 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175452-plasmid-map-sequence-id-353153

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  15. Rab11A[8-175]-His-C Q70L

    Plasmid
    #228042
    Purpose
    Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling. Constitutively active mutation (Q70L).
    Depositor
    Wade Harper
    Insert
    Rab11A[8-175]-His-C Q70L (RAB11A Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Q70L
    Promoter
    Available since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    228042-plasmid-map-sequence-id-450388

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  16. EPB41L3A-c021

    Plasmid
    #175460
    Purpose
    Protein expression in bacterial cells. FERM domain, P107-L390. Used for crystallography (PDB:6IBE).
    Depositor
    Opher Gileadi
    Insert
    EPB41L3 (EPB41L3 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175460-plasmid-map-sequence-id-353154

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  19. pKM3

    Plasmid
    #154168
    Purpose
    Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154168-plasmid-map-sequence-id-309416

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  20. Traptavidin-E6

    Plasmid
    #59549
    Purpose
    pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Core Traptavidin-E6
    Use
    Tags
    E6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  22. CD44I-c011

    Plasmid
    #175449
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175449-plasmid-map-sequence-id-353150

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  25. pST_DHN_VrDHN1a (or YSK2) (pBS0807)

    Plasmid
    #185266
    Purpose
    For the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a (or YSK2). This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    DHN_VrDHN1a (or YSK2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185266-plasmid-map-sequence-id-363793

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  27. DHN_VrDHN1a_mCh-SspB (pBS1074)

    Plasmid
    #185298
    Purpose
    Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    PvLEA4_repeats_k3_26
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185298-plasmid-map-sequence-id-363925

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  30. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    David Christianson
    Article
    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Use
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122174-plasmid-map-sequence-id-237469

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Showing: 101 - 130 of 130 results