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pAAV-CBA-DO(FAS)-GCaMP6s

Plasmid

#110135

Purpose

Cre-off expression of GCaMP6s

Depositor

Bernardo Sabatini

Article

Markowitz et al Cell. 2018 May 10. pii: S0092-8674(18)30

Insert

GCaMP6s

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-SPRTN-E112A

Plasmid

#110215

Purpose

Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.

Depositor

Ivana Marinovic-Terzic, Kristijan Ramadan

Article

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Tags

Flag

Expression

Mammalian

Mutation

E112A, P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCDB364

Plasmid

#110275

Purpose

N-terminal 10-His DsbC TEV-peptide fusion

Depositor

Christopher Bahl

Article

Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

Type

Empty backbone

Tags

10-His, TEV cleavage peptide, and mature DsbC (i.…

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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EGFR D770-N771 insNPG

Plasmid

#11016

Purpose

Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D770-N771 insNPG (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D770-N771 insNPG

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pDGM3B

Plasmid

#110809

Purpose

contains the AAV3B cap genes, AAV2 rep genes, and also adenovirus helper genes

Depositor

David Russell

Article

Khan et al Nat Protoc. 2011 Apr;6(4):482-501. doi:

Insert

AAV3B cap genes, AAV2 rep genes, adenovirus helper genes

Use

AAV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK503

Plasmid

#110616

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pmTurquoise2-LC3

Plasmid

#110947

Purpose

Expresses the autophagosome marker LC3 fused to mTurquoise2

Depositor

Pablo Rivera-Fuentes

Article

Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

mTurquoise2

Expression

Mammalian

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

Plasmid

#110559

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-DIO-intron336-Venus-NLS-D2

Plasmid

#110055

Purpose

Cry1 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and Venus (Cry1 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Promoter

Cry1

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pDXinit

Plasmid

#110101

Purpose

E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE hygro MEN1 WT

Plasmid

#11024

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pSBinit

Plasmid

#110100

Purpose

E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTag

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Tags

myc, His

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-BHLHE40(S243A/E248A)

Plasmid

#110155

Purpose

Expression of human BHLHE40 (S243A/E248A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

Insert

BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)

Tags

HA

Expression

Mammalian

Mutation

S243A/E248A

Promoter

CMV

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pFL

Plasmid

#110739

Purpose

Plasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP site

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Expression

Insect

Promoter

Polyhedrin and p10 promoters

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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EGFR D837A

Plasmid

#11014

Purpose

Retroviral construct for expressing human EGFR with D837A mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D837A (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D837A

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
hCXCR4-LSSmOrange

Plasmid

#110197

Purpose

Encodes for human CXCR4 coding sequence tagged with the LSS-mOrange FP

Depositor

Sergi Padilla Parra

Article

Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

Insert

hCXCR4 (CXCR4 Human)

Tags

LSSmOrange

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR1I2_Hormone-recep

Plasmid

#110020

Purpose

Protein expression and purification of NR1I2_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR1I2_Hormone-recep (NR1I2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-TetON-Sox2

Plasmid

#110280

Purpose

Doxycycline-inducible overexpression of Sox2

Depositor

Trudy Oliver

Article

Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

Insert

SRY (sex determining region Y)-box 2 (Sox2 Mouse)

Use

Lentiviral

Tags

No fusion genes

Expression

Mammalian

Mutation

Wild type

Promoter

TRE

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCK314

Plasmid

#110545

Purpose

Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria

Depositor

John Heap

Article

Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

Inserts

Modified PrhaBAD (CRP-binding sites removed)
yfp
rhaS

Expression

Bacterial

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE3G-FNLS-PGK-Puro

Plasmid

#110847

Purpose

Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

TRE3G

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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