We narrowed to 115 results for: crem

1. S12.1_Empty_Bridge

   Plasmid

   #127670

   Purpose

   Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. S12.2_Bridge + Target

   Plasmid

   #127671

   Purpose

   Ampicillin resistant multiplexing backbone with CRY2HA and mCherry

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Empty Anchor Control

   Plasmid

   #127665

   Purpose

   Encodes for puromycin resistance expressed from the EF1a promoter

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   Puro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Empty Target Control

   Plasmid

   #127667

   Purpose

   Encodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   CRY2-HA-2A-mCherry
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LADL Anchor

   Plasmid

   #127664

   Purpose

   Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   dCas9-CIBN-2A-Puro
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 (D10A,H840A) mutant

   Promoter

   EF1a

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LADL Bridge + Promoter Only Target

   Plasmid

   #127669

   Purpose

   Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   CRY2-HA-2A-mCherry and gRNAs 115 and 117
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and hU6

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Empty Bridge Control Zfp462-Klf4SE

   Plasmid

   #127666

   Purpose

   Encodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   gRNAs 129, 135, 115, 117
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LADL Bridge + Target Zfp462-Klf4SE

   Plasmid

   #127668

   Purpose

   Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and hU6

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCYP15

   Plasmid

   #212848

   Purpose

   Expresses the Micromonospora cremea LRD-modifying cytochrome P450 enzyme

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Insert

   cytochrome P450
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PcymRC

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVH613

    Plasmid

    #169602

    Purpose

    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVH617

    Plasmid

    #169606

    Purpose

    Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (A1-pA::PmPGK1-Fluc-pA::PCREm4-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVH615

    Plasmid

    #169604

    Purpose

    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (A1-pA::PCREm4-Nluc-pA::PmPGK1-Fluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVH616

    Plasmid

    #169605

    Purpose

    Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::A2-pA::PCREm4-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVH614

    Plasmid

    #169603

    Purpose

    Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::PCREm4-Nluc-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX307/PTPN11

    Plasmid

    #140941

    Purpose

    expresses PTPN11

    Depositor

    Kimberly Stegmaier

    Article

    Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

    Insert

    PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLX307/PTPN11 T73I

    Plasmid

    #140939

    Purpose

    expresses PTPN11 T73I

    Depositor

    Kimberly Stegmaier

    Article

    Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

    Insert

    PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    T73I

    Promoter

    EF1a

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLX307/PTPN11 E76G

    Plasmid

    #140862

    Purpose

    expresses PTPN11 E76G

    Depositor

    Kimberly Stegmaier

    Article

    Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

    Insert

    PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E76G

    Promoter

    EF1a

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUWL201PW-creDE

    Plasmid

    #112241

    Purpose

    Streptomyces/E.coli shuttle expression vector encoding creD and creE

    Depositor

    Emily Balskus

    Article

    Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

    Inserts

    creD
    creE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pScPPX2

    Plasmid

    #112877

    Purpose

    Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

    Depositor

    Ursula Jakob, Michael Gray

    Article

    Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

    Insert

    PPX1 (PPX1 Budding Yeast)
    

    Use

    Tags

    6X His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENeGFP PCNAL2 pc653

    Plasmid

    #167564

    Purpose

    The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

    Depositor

    Cristina Cardoso

    Article

    Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge