We narrowed to 5,867 results for: nls

1. pSL1180-HR-PUbEBFP2-NLS

   Plasmid

   #176682

   Purpose

   Expression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Insert

   EBFP2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti poly-ubiquitin (AAEL003888)

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. NLS-SpCas9mSA-His

   Plasmid

   #182951

   Purpose

   Express SpCas9mSA in bacteria

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   NLS-SpCas9mSA-His
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLS-dCas9-trCIB1

   Plasmid

   #64119

   Purpose

   Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   dCas9-trCIB1 fusion
   

   Use

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   dCas9 (D10A H840A, catalytically inactive), trCIB…

   Promoter

   CMV

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. EBFP2-PCNA-19-SV40NLS-4

   Plasmid

   #55250

   Purpose

   Localization: Nucleus, Excitation: 383, Emission: 448

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   3xSV40 NLS and EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. spCas9-NLS-MAV

   Plasmid

   #124212

   Purpose

   SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

   Depositor

   Uri David Akavia

   Article

   Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   His6, MBP, TEV Cleavage site, and two copies of S…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. H_NLS 41kDa (BFP)

   Plasmid

   #201391

   Purpose

   Nucleocytoplasmic transport probe

   Depositor

   Pere Roca-Cusachs

   Insert

   (NLS)SV40-BFP-2PrA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. NLSx3-CRY2PHR-p65

   Plasmid

   #64124

   Purpose

   Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   CRY2PHR-p65
   

   Use

   Tags

   His, NLSx3, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-ICUE3-NLS

   Plasmid

   #138218

   Purpose

   Nuclear-targeted FRET-based cAMP indicator.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   ICUE3-NLS
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol2CG2-QUAS5x:GFPNLS-SV40pA

   Plasmid

   #155122

   Purpose

   Tol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QUAS5x:GFPNLS
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   QUAS5x-E1b

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. NLS-LucWT-GFP

    Plasmid

    #127709

    Purpose

    The expressed ORF encodes nucleus location signal with GFP and WT Luciferase.

    Depositor

    Xiaolu Yang

    Article

    Guo et al J Vis Exp. 2016 Aug 28;(114). doi: 10.37

    Insert

    Luciferase
    

    Use

    Tags

    EGFP and FLAG-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. dCas9-AsLOV2x3-NLSx3

    Plasmid

    #231566

    Purpose

    Expresses components of the LOOMINA optogenetic transcriptional control system.

    Depositor

    Dominik Niopek

    Article

    Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

    Insert

    dCas9-AsLOV2x3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRvTUB-mEGFP-NLS

    Plasmid

    #205011

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
    

    Use

    Tardigrade expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Prab-3::NLS::GFP

    Plasmid

    #68120

    Purpose

    Nuclear Localized GFP for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. GANES-DEVD-BNLS

    Plasmid

    #50842

    Purpose

    Expresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A and ddGFP B
    

    Use

    Tags

    NES sequence LALKLAGLDIGS placed after ddGFP A an…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3_N-DYK_MTHFD1-NLS

    Plasmid

    #133023

    Purpose

    NLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

    Depositor

    Stefan Kubicek

    Article

    Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

    Insert

    MTHFD1 (MTHFD1 Human)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. epB_CAG_TetRFlag-nls-tdTom_DEx4

    Plasmid

    #119909

    Purpose

    ePiggyBac mammalian expression plasmid for TetR-tdTom fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    TetRFlag-nls-tdTom
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Prab-3::NLS::GCaMP6s

    Plasmid

    #68119

    Purpose

    Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    rab-3::NLS::GCaMP6s
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-3xnls-Tq-Ca-FLITS

    Plasmid

    #145030

    Purpose

    piggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    3xnls-Tq-Ca-FLITS
    

    Use

    Tags

    3xnls-Tq-Ca-FLITS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLBS.EF NLS-pHlu

    Plasmid

    #129324

    Purpose

    pH measurement, nucleus, lentivirus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    pHluorin (from H. Imamura)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+ EGFP-3NLS

    Plasmid

    #165400

    Purpose

    Expresses nuclear localized EGFP

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Insert

    Nuclear Localization Signal
    

    Use

    Avian, xenopus, zebrafish

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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