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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 4,504 results for: pcr

Showing: 871 - 900 of 4504 results

  2. IpO

    Plasmid
    #48233
    Purpose
    Base vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.
    Depositor
    Ronald Davis
    Article
    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10
    Inserts
    URA3 3' fragment
    URA3 5' fragment
    Use
    Routine cloning vector
    Tags
    Expression
    Mutation
    -242 upstream to URA3 ORF +495 and URA3 ORF from …
    Promoter
    URA3 promoter and none
    Available since
    Feb. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    48233-plasmid-map-sequence-id-69537

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  7. pRB17

    Plasmid
    #52527
    Purpose
    serves as template for PCR-mediated fusion of U6-promoter with sgRNA
    Depositor
    Klaus Foerstemann
    Article
    Bottcher et al Nucleic Acids Res. 2014 Apr 19.
    Insert
    Drosophila U6C promoter
    Use
    CRISPR; Blunt cloning kit
    Tags
    T7-promoter
    Expression
    Insect
    Mutation
    Promoter
    Available since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    52527-plasmid-map-sequence-id-136307

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  9. pJH124

    Plasmid
    #48259
    Purpose
    Template plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.
    Depositor
    Ronald Davis
    Article
    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10
    Inserts
    URA3 3' fragment
    URA3 5' fragment
    Ag TEF1 promoter
    Use
    Routine cloning vector
    Tags
    Expression
    Mutation
    -242 upstream to URA3 ORF +495 and URA3 ORF from …
    Promoter
    Ag TEF1 promoter
    Available since
    Feb. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    48259-plasmid-map-sequence-id-69693

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  11. pOpen-ETSSB

    Plasmid
    #165549
    Purpose
    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    Extreme Thermostable Single-Stranded DNA Binding Protein
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165549-plasmid-map-sequence-id-339430

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  12. pOpen-BovDNTT

    Plasmid
    #165580
    Purpose
    Adds dT at the end of PCR blunted DNA fragments/vectors that can then be used in TOPO cloning; Addition of homopolymer tails to the 3' ends of DNA; Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP); TUNEL assay (in situ localization of apoptosis); TdT dependent PCR
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    Bovine DNTT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 28, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165580-plasmid-map-sequence-id-338558

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  13. pEasyG3_nat

    Plasmid
    #184918
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184918-plasmid-map-sequence-id-368408

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  14. pEasyG3_mic

    Plasmid
    #184920
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184920-plasmid-map-sequence-id-368413

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  15. pEasyG3_hph

    Plasmid
    #184919
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184919-plasmid-map-sequence-id-368411

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  16. pEasyG2_zeo

    Plasmid
    #184913
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184913-plasmid-map-sequence-id-379508

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  17. pEasyG2_nat

    Plasmid
    #184914
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184914-plasmid-map-sequence-id-367949

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  18. pEasyG2_hph

    Plasmid
    #184915
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184915-plasmid-map-sequence-id-367948

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  19. pEasyG2_mic

    Plasmid
    #184916
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184916-plasmid-map-sequence-id-371408

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  20. pEasyG3_zeo

    Plasmid
    #184917
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184917-plasmid-map-sequence-id-368415

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  21. pOpen-T4 g32

    Plasmid
    #165560
    Purpose
    A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    T4 gene 32
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165560-plasmid-map-sequence-id-339953

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  23. pSKAP_AT

    Plasmid
    #122722
    Purpose
    pSKAP plasmid containing the unique DNA barcode "AT" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
    Depositor
    Travis Bourret
    Article
    Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37
    Insert
    Barcode AT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    122722-plasmid-map-sequence-id-245021

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  24. pSKAP_AS

    Plasmid
    #122721
    Purpose
    pSKAP plasmid containing the unique DNA barcode "AS" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
    Depositor
    Travis Bourret
    Article
    Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37
    Insert
    Barcode AS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    122721-plasmid-map-sequence-id-245019

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  25. pSKAP_AN

    Plasmid
    #122716
    Purpose
    pSKAP plasmid containing the unique DNA barcode "AN" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
    Depositor
    Travis Bourret
    Article
    Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37
    Insert
    Barcode AN
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    122716-plasmid-map-sequence-id-245013

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Showing: 871 - 900 of 4504 results