Skip to main content
Addgene
Showing: 1451 - 1500 of 2318 results

  1. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

    Plasmid
    #185061
    Purpose
    Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185061-plasmid-map-sequence-id-376807

    Enlarge

  2. TJP3

    Plasmid
    #25310
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TJP3:G508-L808:cH6 (TJP3 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids G508-L808
    Promoter
    Available since
    April 4, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. pLV-Enh eGFP Reporter-muKC2-IGR4

    Plasmid
    #59273
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Intergenic 3kb downstream Krt82
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pLV-Enh eGFP Reporter-muKC2-IGR7

    Plasmid
    #59276
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    intergenic 4kb upstream krt1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pLV-Enh eGFP Reporter-muKC2-IGR8

    Plasmid
    #59277
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Intergenic 2kb downstream krt79
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pLV-Enh eGFP Reporter-muKC2-IGR1

    Plasmid
    #59270
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Intergenic 20kb downstream krt80
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pLV-Enh eGFP Reporter-muKC2-IGR2

    Plasmid
    #59271
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Intergenic 10kb upstream krt80
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pLV-Enh eGFP Reporter-huKC1-IGR19

    Plasmid
    #59285
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the human keratin type I cluster.
    Depositor
    Benjamin Yu
    Insert
    9kb upstream hKrt14-1700
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pLV-Enh eGFP Reporter-muKC2-IGR6

    Plasmid
    #59275
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    intergenic 11kb upstream krt2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pLV-Enh eGFP Reporter-muKC2-IGR3

    Plasmid
    #59272
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Intergenic 4kb downstream Gm6042
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pLV-Enh eGFP Reporter-muKC2-IGR11

    Plasmid
    #59280
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Intergenic 2kb upstream krt18
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pIA035

    Plasmid
    #200426
    Purpose
    amyE::(Ppen-lacIΔ11-dronPA cm). PALM integration vector includes dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Use
    Tags
    dronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Ppen
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    200426-plasmid-map-sequence-id-405336

    Enlarge

  18. pIA027

    Plasmid
    #200418
    Purpose
    amyE::(dronPA cm), for dronPA fusions. PALM-compatible integration vector for Bacillus subtilis. Includes dronPA photoswitchable FP with multi cloning sites (MCS) for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Tags
    dronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200418-plasmid-map-sequence-id-405455

    Enlarge

  19. pGL3-pSMANTIS_1kb_1 KLF2mut 449,899

    Plasmid
    #194198
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,899)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    2 mutated KLF binding sites (position 449,899)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194198-plasmid-map-sequence-id-381969

    Enlarge

  20. pGL3-pSMANTIS_1kb_1 KLF2mut 449,548

    Plasmid
    #194199
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,548)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    2 mutated KLF binding sites (position 449,548)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194199-plasmid-map-sequence-id-381923

    Enlarge

  21. pGL3-pSMANTIS_1kb_1 KLF2mut 449,732

    Plasmid
    #194200
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,732)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    2 mutated KLF binding sites (position 449,732)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194200-plasmid-map-sequence-id-381961

    Enlarge

  22. pGL3-pSMANTIS_1kb_1 KLF2mut 449,884

    Plasmid
    #194201
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,884)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    2 mutated KLF binding sites (position 449,884)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194201-plasmid-map-sequence-id-381985

    Enlarge

  24. pLSLGFP

    Plasmid
    #51494
    Purpose
    pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    GFP
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Tags
    NLS and flag
    Expression
    Mutation
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51494-plasmid-map-sequence-id-78590

    Enlarge

  25. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

    Enlarge

  28. pBS513 EF1alpha-cre

    Plasmid
    #11918
    Purpose
    Expresses Cre under the control of the elongation factor-1 alpha (EF1alpha) promoter; includes upstream Kozak modification to increase expression.
    Depositor
    Brian Sauer
    Article
    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.
    Insert
    cre (cre bacteriophage P1)
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    March 16, 2007
    Availability
    Academic Institutions and Nonprofits only
    11918-plasmid-map-sequence-id-163454

    Enlarge

  29. pIA030

    Plasmid
    #200424
    Purpose
    thrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Use
    Tags
    PAmCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Ppen
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    200424-plasmid-map-sequence-id-405349

    Enlarge

  35. pcDNA3.1-Beta2-PAmcherry1

    Plasmid
    #114190
    Purpose
    studying clustering of Beta2 receptors by superresolution microscopy (PALM)
    Depositor
    Aleksandra Radenovic
    Insert
    Adrenergic receptor beta 2 (ADRB2 Human)
    Use
    Tags
    3HA, FLAG, and PAmcherry1
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114190-plasmid-map-sequence-id-224309

    Enlarge

  36. CD44MMA-c002

    Plasmid
    #175452
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, Q23-D172. Used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    Cd44 (Cd44 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175452-plasmid-map-sequence-id-353153

    Enlarge

  37. pHBS1202 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 4xΦ]

    Plasmid
    #107845
    Purpose
    To test the effect of sequence on TDP43 splicing activity
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All hydrophobics combined in four clusters in spl…
    Promoter
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107845-plasmid-map-sequence-id-203697

    Enlarge

  38. pHBS1203 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 2xΦ]

    Plasmid
    #107844
    Purpose
    To test the effect of sequence on TDP43 splicing activity
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All hydrophobics combined in two clusters in spli…
    Promoter
    Available since
    April 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    107844-plasmid-map-sequence-id-203696

    Enlarge

  40. pIA036

    Plasmid
    #200425
    Purpose
    amyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Use
    Tags
    bsdronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Ppen
    Available since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    200425-plasmid-map-sequence-id-443100

    Enlarge

  42. pHBS1201 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 6xΦ]

    Plasmid
    #107846
    Purpose
    To test the effect of sequence on TDP43 splicing activity
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All hydrophobics combined in six clusters in spli…
    Promoter
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107846-plasmid-map-sequence-id-203698

    Enlarge

  50. NXPE1_pcDNA3.4-TOPO

    Plasmid
    #226841
    Purpose
    Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.
    Depositor
    Bert Vogelstein
    Insert
    NXPE1 (NXPE1 Human)
    Use
    Tags
    x6 HIS and an IL2 secretion signal both N termina…
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    226841-plasmid-map-sequence-id-456206

    Enlarge

Showing: 1451 - 1500 of 2318 results