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pAAV-CAG-SuperNovaGreen-T2A-dTomato

Plasmid

#193798

Purpose

Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

SuperNova Green

Use

AAV

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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sgLenti-orthogonal

Plasmid

#105997

Purpose

Dual Streptococcus pyogenes and Staphylococcus aureus sgRNA expression vector

Depositor

Mike McManus

Article

Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

Type

Empty backbone

Use

CRISPR and Lentiviral; S.pyogenes sgrna expressio…

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST_FUS_G2A_R521H

Plasmid

#26381

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

R521H

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pDEST_FUS_C2G_R521G

Plasmid

#26380

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

R521G

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pDEST_TO_FUS

Plasmid

#26373

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG-T2A-dTomato

Plasmid

#193796

Purpose

Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST8_FLAGHA_FUS_HIS

Plasmid

#26375

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Use

Tags

FLAG/HA and His

Expression

Insect

Mutation

delta 527 (STOP codon)

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#164046

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA gene

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

mTagBFG-TriTag

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Gata1

Plasmid

#41085

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Gata1 (Gata1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
pMIR-Dsred-IRES-His Keap

Plasmid

#62457

Purpose

expresses his tagged human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Use

Tags

HisTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCA24-pHR-CMV-stdMCP-p65-HSF1-T2A-GFP

Plasmid

#199455

Purpose

Expression of stdMCP-PH in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

stdMCP-PH

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCA25-pHR-CMV-stdPCP-p65-HSF1-T2A-GFP

Plasmid

#199456

Purpose

Expression of stdPCP-PH in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

stdPCP-PH

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR4_FUS_C2G_R521G

Plasmid

#26369

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Use

Entry vector

Tags

Expression

Mutation

R521G

Promoter

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
Zebrafish-gRNA-0002

Plasmid

#42242

Purpose

gRNA targeted to zebrafish gene drd3

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-drd3 (drd3 Zebrafish)

Use

CRISPR; Zebrafish expression

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
pENTR4_FUS_G2A_R521H

Plasmid

#26370

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Use

Entry vector

Tags

Expression

Mutation

R521H

Promoter

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pLVX-shRNA2-zsGreen-PGK-puro_HSALNG0104472-163

Plasmid

#197902

Purpose

For the stable knockdown experiments using shRNA, a complementary sequence (HSALNG0104472-163) targeting the HSALNG0104472 transcript was inserted.

Depositor

Bo Wang

Article

Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

Insert

HSALNG0104472-163 (HSALNG0104472-scramble-sequence)

Use

Lentiviral

Tags

zsGreen1

Expression

Mammalian

Mutation

15q11.2 deletion

Promoter

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCA23-sgRNA vector-U6-sgTS2 (SpCas9)

Plasmid

#199454

Purpose

U6-driven sgRNA targeting TS2 sequence (GGAGCTTACTGAGACTCTTC)

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TS2 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

mouse U6

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCA28-pMa-PspCas13b crRNA-TS1

Plasmid

#199459

Purpose

U6-driven crRNA targeting TS1 sequence (CCTCCTCGGAGAGCATCGGTGC )

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TS1 crRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

mouse U6

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCA17-CRISPR-Tag (11XTS1) for dSpCas9

Plasmid

#199448

Purpose

CRISPR-Tag sequence that can be efficiently bound by dSpCas9

Depositor

Baohui Chen

Article

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

CRISPR-Tag

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCA29-pCS2-CMV-Zebrafish NarTag (eGFP)

Plasmid

#199460

Purpose

Expression of Zebrafish NarTag (eGFP harbors 9XMS2 in its intron) in zebrafish embryos

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

Zebrafish NarTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge