-
-
pKD001 pME minprom_EGFP
Plasmid#195955PurposeGateway pME fluorophoreDepositorInsertmouse beta-globin minimal promoter EGFP
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-mCherry-Rheb(C181S)
Plasmid#192438PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.DepositorInsertLyso-mCherry-Rheb(C181S) (RHEB Human)
UseTagsFull-length LAMP1 and mCherryExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(C181S)
Plasmid#192437PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKH3-TRIM28
Plasmid#45569DepositorInsertTRIM28 (TRIM28 Human)
UseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
ePop
Plasmid#50953PurposeExpresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillationsDepositorInsertsE lysis protein
LuxI
LuxR
UseTagsExpressionMutationEarly stop codon introduced in mutated luxR resul…PromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Halo-TEV-Nrf2
Plasmid#62455PurposeHalo-His6 tagged Nrf2 for E.coli expressionDepositorInsertNrf2 (NFE2L2 Human)
UseTagsHis6-Halo-TEVExpressionBacterialMutationPromoterAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-FLNa S2152A
Plasmid#8983DepositorInsertFilamin A S2152A (FLNA Human)
UseTagsmycExpressionMammalianMutationSer2152AlaPromoterAvailable sinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-Tpr
Plasmid#60882PurposeExpresses Flag-Tagged nucleoporin Tpr in Mammalian CellsDepositorInsertTpr (TPR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TRIM28
Plasmid#45568DepositorInsertTRIM28 (TRIM28 Human)
UseTagsEGFPExpressionMammalianMutationG34S mutation in EGFPPromoterCMVAvailable sinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
PF4-bio-His
Plasmid#53411PurposeExpresses full-length Platelet factor 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPF4 (PF4 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-TRIM28
Plasmid#45570DepositorInsertTRIM28 (TRIM28 Human)
UseTagsGSTExpressionBacterialMutationPromoterPtacAvailable sinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAIP-hXRCC1pd-eGFP
Plasmid#206033PurposeMammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoterDepositorInserthXRCC1
UseTagseGFPExpressionMammalianMutationS103A,R186A,S184A,S193A,S219A,S220A,S236A,S268APromoterCAGAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S
Plasmid#62458Purposeexpresses his-tagged C151S human Keap1 proteinDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-Halo-TevExpressionMammalianMutationChanged Cysteine 151 of Keap1 to serinePromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-myrAkt
Plasmid#49267Purposeretroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequenceDepositorInsertAkt (AKT1 Bovine)
UseRetroviralTagsIRES-EGFP and myrExpressionMammalianMutationN2D, D149E and A478G compared to AAW71957.1PromoterAvailable sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
145-pGL3ctrl-3' UTR
Plasmid#28175DepositorInsertLuciferase flanked by tp53 UTRs (TP53 Human)
UseLuciferaseTagsExpressionMammalianMutationNote that insert length is based on sum of the 2 …PromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S
Plasmid#62459Purposeexpresses his-tagged C288S human Keap1 proteinDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-Halo-TevExpressionMammalianMutationChanged Cysteine 288 of Keap1 to serinePromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDest-636
Plasmid#159574PurposeGateway Destination vector for protein expression in baculovirusDepositorTypeEmpty backboneUseTagsHis6-MBPExpressionInsectMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
PEAR1-bio-His
Plasmid#51860PurposeExpresses full-length Platelet endothelial aggregation receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPEAR1 (PEAR1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuro2-UBXD1-mCherry
Plasmid#31835DepositorInsertUBXD1 (UBXN6 Human)
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only
