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We narrowed to 777 results for: PI

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Showing: 581 - 600 of 777 results

  1. pTE3329

    Plasmid
    #107538
    Purpose
    Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Lb crRNA
    hLbCpf1(RR mutant)
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    G532R, K595R
    Promoter
    CMV and human U6
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107538-plasmid-map-sequence-id-207235

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  2. pTE3334

    Plasmid
    #107537
    Purpose
    Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Mb crRNA
    hMbCpf1(RVR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N576R, K582V, N586R
    Promoter
    CMV and human U6
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107537-plasmid-map-sequence-id-207346

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  3. pTE3330

    Plasmid
    #107534
    Purpose
    Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Lb crRNA
    hLbCpf1(RVR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    G532R, K538V, Y542R
    Promoter
    CMV and human U6
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107534-plasmid-map-sequence-id-207314

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  4. pTE3336

    Plasmid
    #107541
    Purpose
    Expresses Mb crRNA and human codon optimized MbCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Mb crRNA
    hMbCpf1(RR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N576R, K637R
    Promoter
    CMV and human U6
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107541-plasmid-map-sequence-id-207245

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  5. pTE3333

    Plasmid
    #107540
    Purpose
    Expresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Fn crRNA
    hFnCpf1(RR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N607R, K671R
    Promoter
    CMV and human U6
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107540-plasmid-map-sequence-id-207239

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  6. pTE3331

    Plasmid
    #107536
    Purpose
    Expresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Fn crRNA
    hFnCpf1(RVR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N607R, K613V, N617R
    Promoter
    CMV and human U6
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107536-plasmid-map-sequence-id-207338

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  8. B-HypaR-SpCas9

    Plasmid
    #126764
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-Hypa2SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A; N692A; M694A; Q695A; H698A; amino acids 10…
    Promoter
    CBh
    Available Since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    126764-plasmid-map-sequence-id-410886

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  11. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-498727

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  12. pcDNA3.3-Nm-dCas9-p300 Core

    Plasmid
    #61365
    Purpose
    encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    Nm-dCas9-p300 Core (EP300 Human, Synthetic, Neisseria meningiditis)
    Use
    CRISPR and Synthetic Biology
    Tags
    HA tag and NLS
    Expression
    Mammalian
    Mutation
    D16A, D587A, H588A, N611A in Nm Cas9
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61365-plasmid-map-sequence-id-244292

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  13. pSc1-DD

    Plasmid
    #80439
    Purpose
    Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Tags
    E. coli dihydrofolate reductase
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available Since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80439-plasmid-map-sequence-id-151455

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  14. pSc1-puro

    Plasmid
    #80438
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Puromycin resistance
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV, U6, and hPGK
    Available Since
    Sept. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    80438-plasmid-map-sequence-id-151453

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  18. ADH4

    Plasmid
    #38910
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ADH4 (ADH4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pCdc45-mCherry

    Plasmid
    #211380
    Purpose
    mammalian expression of human Cdc45 C-terminally fused to mCherry
    Depositor
    Helmut Pospiech
    Insert
    Cdc45 (CDC45 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211380-plasmid-map-sequence-id-420495

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  20. pUC19_CDC45_dTAG

    Plasmid
    #232733
    Purpose
    Generates CDC45 dTAG C ternimal knock-in cell lines as donor
    Depositor
    Junjie Chen
    Insert
    CDC45 (CDC45 Human)
    Expression
    Bacterial
    Mutation
    Synonymous mutation
    Available Since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    232733-plasmid-map-sequence-id-465446

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Showing: 581 - 600 of 777 results