Addgene

We narrowed to 2,911 results for: PPL

Showing: 581 - 600 of 2911 results

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pLZ12Km2-P23R:TA:Xen5

Plasmid

#88902

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + LuxABCDE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT EGFP-miniAID

Plasmid

#101714

Purpose

For amplification of a EGFP-miniAID for N-terminal tagging

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

EGFP-miniAID

Use

Tags

EGFP-miniAID

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pWS3805

Plasmid

#185746

Purpose

Sub-array spacer 1/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 1/4

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pWS3806

Plasmid

#185747

Purpose

Sub-array spacer 2/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 2/4

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCM433kanT

Plasmid

#61262

Purpose

Sucrose counterselection (sacB) vector for unmarked allelic exchange.

Depositor

Mary Lidstrom

Article

Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

Type

Empty backbone

Use

Synthetic Biology; Sucrose counterselection

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pBBR1-kan-Ptet-plu-gamma-ET_rhi145

Plasmid

#180427

Purpose

Genome engineering of Agrobacterium

Depositor

Jun Fu

Article

Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

Insert

plu-gamma-ET_rhi145

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptet1

Plasmid

#218360

Purpose

Empty backbone with tetR-PtetA expression system with single tetR operator site

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-c2E

Plasmid

#75291

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Use

Tags

maltose-binding protein

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pWS3808

Plasmid

#185749

Purpose

Sub-array spacer 4/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 4/4

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_Z17-ehaA_LacI

Plasmid

#186313

Purpose

Plasmid for inducible E coli expression of the Z17 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

Z17-ehaA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pWS3807

Plasmid

#185748

Purpose

Sub-array spacer 3/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 3/4

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBBR1-kan-Ptet-plu-gamma-ETh_rhi483

Plasmid

#180429

Purpose

Genome engineering of Agrobacterium

Depositor

Jun Fu

Article

Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

Insert

plu-gamma-ETh_rhi483

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_Z18-ehaA_LacI

Plasmid

#186314

Purpose

Plasmid for inducible E coli expression of the Z18 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

Z18-ehaA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph

Plasmid

#218366

Purpose

Empty backbone for Tn7-site insertion

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB

Plasmid

#30505

Depositor

Ichiro Matsumura

Article

Murin et al Appl Environ Microbiol. 2011 Oct 21.

Insert

beta-glucuronidase (gusA Escherichia coli)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pRGD-KmR

Plasmid

#74108

Purpose

Plasmid containing kanamycin resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

aphII

Use

Bacterial vector based on pmb1 replicon

Tags

Expression

Mutation

Promoter

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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V308 pDNR MCS lacZ alpha - Donor

Plasmid

#11691

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Donor vector

Tags

Expression

Mutation

Promoter

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pTETSIX

Plasmid

#74104

Purpose

mCherry-encoding vector for markerless deletion mutagenesis in Bradyrhizobium diazoefficiens and other Alphaproteobacteria

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

tetA(C)

Use

Conjugative bacterial vector

Tags

Expression

Mutation

C-to-T transition at pos. no. 1049 of tetA(C) lea…

Promoter

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pQMD

Plasmid

#48103

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of myc-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Use

Tags

myc tag

Expression

Bacterial

Mutation

Promoter

PQ5

Available since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pFlare5GdP

Plasmid

#90250

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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