We narrowed to 945 results for: dcas9, grna

1. THI VP64 pGAP T3

   Plasmid

   #161490

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T3_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting T3 in the THI11 promoter)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. THI VP64 pGAP T4

   Plasmid

   #161491

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T4_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting T4 in the THI11 promoter)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. THI VP64 pALD4 T1

   Plasmid

   #161482

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T1_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (targeting T1 of pTHI11 promoter)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pALD4, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. THI VP64 pALD4 T2

   Plasmid

   #161483

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T2_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (targeting T2 of pTHI11 promoter)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pALD4, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. THI VP64 pALD4 T3

   Plasmid

   #161484

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T3_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (targeting T3 of pTHI11 promoter)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pALD4, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MT-AvrXa10-FT

   Plasmid

   #234373

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus

   Depositor

   Michael Smanski

   Article

   Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202

   Inserts

   NbGP41-GFP-AvrXa10-GB1
   dCas9-24XGP41
   gRNAs targeting FT locus
   RFP
   

   Use

   Synthetic Biology

   Tags

   GB1, GFP, and GP41

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT077

   Plasmid

   #137879

   Purpose

   Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT076

   Plasmid

   #137880

   Purpose

   Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAPH07

   Plasmid

   #234345

   Purpose

   Library-scale IPTG-inducible gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK

   Depositor

   Jerome Fox

   Article

   Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12

   Insert

   Type II dCas9 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPRi_Mxi1_yl

    Plasmid

    #91248

    Purpose

    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAPH021

    Plasmid

    #234346

    Purpose

    Library-scale IPTG-inducible single transcript dual-gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK

    Depositor

    Jerome Fox

    Article

    Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12

    Inserts

    Type II dCas9 sgRNA
    Type II dCas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. THI ONLY pALD4 T-

    Plasmid

    #161497

    Purpose

    Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

    Plasmid

    #84258

    Purpose

    Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    Sp sgTRE3G
    PYL1-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    IRES-mCherry and PYL1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and mouse U6

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

    Plasmid

    #84260

    Purpose

    Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    Sp sgSV40
    PYL1-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    IRES-mCherry and PYL1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and mouse U6

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK412

    Plasmid

    #155371

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSH-Csy4-T2A-SpRFN

    Plasmid

    #85754

    Purpose

    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Insert

    Csy4-T2A-FokI-dCas9
    

    Use

    CRISPR

    Tags

    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPathBrick

    Plasmid

    #65006

    Purpose

    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive native promoters

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. GG-dest

    Plasmid

    #69538

    Purpose

    Destination plasmid for gRNA concatenation Golden Gate cloning

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCD296.7

    Plasmid

    #113320

    Purpose

    dCas9, MCP-TetD, J107 scRNA.b1

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    dCas9, MCP-TetD, J107 scRNA.b1_MS2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCD294.9

    Plasmid

    #113319

    Purpose

    dCas9, PCP-SoxS_R93A, J109 scRNA.b1

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    dCas9, PCP-SoxS_R93A, J109 scRNA.b1_PP7
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    R93A

    Promoter

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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