Addgene

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pEK36

Plasmid

#210598

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter

Use

Tags

mClover3

Expression

Bacterial and Yeast

Mutation

Promoter

SpH2Bpro

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEK22

Plasmid

#210588

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdH3pro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Mutation

Promoter

BdH3pro

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGI3EM17

Plasmid

#210569

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Mutation

Promoter

SpH2Bpro

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pV1F(Spec)

Plasmid

#205086

Purpose

Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

Depositor

Suomeng Dong

Article

Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

Insert

AtVIP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-zeo largeTgenomic

Plasmid

#1778

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T and small T antigen (SV40gp6 simian virus)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

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pV1F

Plasmid

#205084

Purpose

Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

Depositor

Suomeng Dong

Article

Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

Insert

AtVIP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

agorbacterium virB operon promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pZY102

Plasmid

#73933

Purpose

This plasmid was derived from pZY101 by inserting a GUS-intron reporter gene cassette into multiple cloning sites. This plasmid is recommended for use as empty control for service event request.

Depositor

Zhanyuan Zhang

Article

Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

Inserts

bar
GUS

Use

Tags

Expression

Plant

Mutation

intron is inserted into the GUS coding region

Promoter

35S

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pTA7002-avrPto

Plasmid

#49156

Purpose

Plant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPto

Depositor

Sheng Yang He

Article

Hauck et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Insert

avrPto

Use

Plant transformation binary vector

Tags

Expression

Mutation

Promoter

dexamethasone inducible

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pRK5 TGF beta type I receptor Flag

Plasmid

#14831

Depositor

Rik Derynck

Article

Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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Hmga2 3'UTR wt luciferase (Luc-wt)

Plasmid

#14785

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Luc-wt (Hmga2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro Rb shRNA

Plasmid

#10670

Depositor

William Hahn

Article

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

Insert

Rb shRNA (RB1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pKL2013

Plasmid

#172182

Purpose

A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.

Depositor

Kan Wang

Article

McCaw et al Front Genome Ed. 2021 Jan 11;2:622227. d

Insert

mCherry

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRN119

Plasmid

#176884

Purpose

Encodes STM and WUS2 for ectopic meristem induction in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV:Luc+:HSP, Nos::WUS2:pinII, and 35:STM:35S

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-3aHA

Plasmid

#18030

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3a (Wnt3a Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

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Ubiq-Cas9.874W

Plasmid

#112686

Purpose

Express hSpCas9 under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Use

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

Ubiquitin-63E promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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vas-Cas9.874Z

Plasmid

#112687

Purpose

Express hSpCas9 under vasa promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Use

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

vasa

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGI3EM09

Plasmid

#135482

Purpose

Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunHsp70pr hph-GFP ADH1t

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

SpunHsp70pr

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pKB01-PssbB-mKate2

Plasmid

#69105

Purpose

Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

mKate2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pGI3EM18

Plasmid

#135484

Purpose

Agrobacterium T-DNA with SpunH2Bpr driving hph-tdTomato

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunH2Bpr hph-tdTomato ADH1t

Use

Tags

tdTomato

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2Bpr

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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nos-Cas9.874Z1

Plasmid

#112685

Purpose

Express hSpCas9 under nanos promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Use

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

nanos

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge