We narrowed to 14,438 results for: NTS

1. pUb dRxCas13d + RxCas13d guide RNA

   Plasmid

   #176304

   Purpose

   Expresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   RxCas13d
   

   Tags

   HA tag

   Expression

   Insect

   Mutation

   R239A, H244A, R858A, H863A

   Promoter

   Ubi-p63e

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe-PI(WT)-Src(YF)-mCherry

   Plasmid

   #87357

   Purpose

   PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   Src isoform 2 (Src Mouse)
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Y535F

   Promoter

   CMV

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSUPER IKKalpha

   Plasmid

   #26208

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   pSUPER IKK alpha (CHUK Human)
   

   Use

   RNAi

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

4. (326) pAAV SV40-ZsGreen U6-gRNA

   Plasmid

   #163020

   Purpose

   Fluorescent reporter for Sa gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Zs Green
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-BE4-RrA3F F130L

   Plasmid

   #138341

   Purpose

   Mammalian expression plasmid for BE4-RrA3F F130L

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-RrA3F F130L
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. GW1-mito-mCherryEA

   Plasmid

   #207868

   Purpose

   Mitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

   Depositor

   Mathew Tantama

   Article

   Rajendran et al ACS Omega. 2018 Aug 31;3(8):9476-9486. d

   Insert

   mito-mCherryEA
   

   Tags

   4xCox8 mitochondrial targeting

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMP71-tCD19-LLO-SIINFEKL

   Plasmid

   #174598

   Purpose

   expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and SIINFEKL epitopes

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   membrane bound CD19, LLO antigen, SIINFEKL (ovalbumin) antigen
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEE531

   Plasmid

   #149290

   Purpose

   T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/FRT/TO_H2B_HaloTag9

   Plasmid

   #169334

   Purpose

   Nuclear expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag9 (H2BC21 Human)
   

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGE670

    Plasmid

    #153236

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-NtLAtC60αβC20

    Plasmid

    #160873

    Purpose

    Expression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Expression

    Bacterial

    Promoter

    rbcL: pBAD, chaperones: T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGE463

    Plasmid

    #153234

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA15-Ubc-NLS- scFV-tdTomato

    Plasmid

    #199446

    Purpose

    Expression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells

    Depositor

    Baohui Chen

    Article

    Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

    Insert

    scFV-tdTomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    human ubiquitin C (UBC)

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Erk1

    Plasmid

    #29578

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    Erk1 (MAPK3 Human)
    

    Tags

    FLAG, HIS6, and TEV

    Expression

    Bacterial

    Mutation

    K289N mutation present. Starting Met removed from…

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1

    Plasmid

    #46676

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-133a-1 (MIR133A1 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro

    Plasmid

    #31480

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (ani target)
    

    Use

    Lentiviral

    Mutation

    mCherry has M9S, M16L to reduce background mCherr…

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFAB815

    Plasmid

    #47854

    Purpose

    BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    rnpB T1 terminator
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLTetO9

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTX209

    Plasmid

    #89269

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsPDS-gRNA01 and OsPDS-gRNA02
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Promoter

    Maize Ubiquitin1 promoter

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. GalT-moxDendra2

    Plasmid

    #89789

    Purpose

    mammalian expression of GC localized moxDendra2

    Depositor

    Erik Snapp

    Article

    Kaberniuk et al Chem Commun (Camb). 2017 Feb 9;53(13):21

    Insert

    GalT signal anchor transmembrane domain (B4GALT1 Human)
    

    Tags

    moxDendra2

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. KHBD00222

    Plasmid

    #34370

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG1832 (CG1832 Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only