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pLKO.1-puro-cfRab11a_2

Plasmid

#26710

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab11a RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rab11a RNAi.

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pOGG008

Plasmid

#113982

Purpose

Vector construction module. Kanamycin/neomycin resistance module for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Kanamycin/neomycin

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG006

Plasmid

#113981

Purpose

Golden Gate Level 2 cloning site, position 1 module for vector construction

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Level 2 golden gate cloning vector

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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MTK5_009

Plasmid

#123853

Purpose

Encodes ConR6 as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector R6

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK5_010

Plasmid

#123854

Purpose

Encodes ConR7 as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector R7

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-PRLH

Plasmid

#184618

Purpose

For expression of a fluorescent sensor for Prolactin-releasing peptide factor in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-PRLH

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-NOC

Plasmid

#184611

Purpose

For expression of a fluorescent sensor for nociceptin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NOC

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pQE30-HaAEP1

Plasmid

#159530

Purpose

Expresses sunflower (Helianthus annuus) asparaginyl endopeptidase 1 in E. coli

Depositor

Joshua Mylne

Article

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Helianthus annuus asparaginyl endopeptidase 1 (LOC110922574 Synthetic)

Tags

Six-Histidine tag

Expression

Bacterial

Promoter

T5

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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MTK4a_003

Plasmid

#123817

Purpose

Encodes Nuclear import sequence as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

NLS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_L4(EAAAK)3

Plasmid

#131211

Purpose

Cloning vector containing A-B-C casette with linker L4 in site B

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

(EAAAK)3 linker

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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p6672 MSCV-IP N-HAonly 52E6

Plasmid

#44136

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV52 E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA HA FAK Y577F

Plasmid

#50507

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y577F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pBS NFATC1

Plasmid

#19203

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

NFATC1 (NFATC1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBW809_pCAG-PV6-F3-mCherry-BGHpA

Plasmid

#87585

Purpose

PV6 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pT7T-CcadTSMod

Plasmid

#99874

Purpose

RNA synthesis for microinjection into xenopus laevis embryo

Depositor

Marc Tramier

Article

Herbomel et al Sci Rep. 2017 Mar 22;7:45058. doi: 10.10

Insert

CcadTSMod

Use

Xenopus microinjection

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shMTH1-2

Plasmid

#21298

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

shMTH1-2 (NUDT1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pDest472 SHP-1 S591A

Plasmid

#21805

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Tags

GFP

Expression

Mammalian

Mutation

Serine 591 changed to Alanine

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
pLO101

Plasmid

#90221

Purpose

Complements mutations in Aspergillus nidulans gene biA (AN6644) (mutants require biotin)

Depositor

Berl Oakley

Article

Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

Insert

ATET_07102

Use

Aspergillus

Promoter

pLac

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLO102

Plasmid

#90222

Purpose

Complements mutations in Aspergillus nidulans gene choA (AN2154) (mutants require choline)

Depositor

Berl Oakley

Article

Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

Insert

ATET_02347

Use

Aspergillus

Promoter

pLac

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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YN2_1_LT84_RPL13A

Plasmid

#177712

Purpose

Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.NgCAM-Halo

Plasmid

#162719

Purpose

Mammalian expression of chicken NgCam

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

NgCam (L1CAM Chicken)

Tags

Halo

Expression

Mammalian

Mutation

E137K, K138E, and K642E

Promoter

Chicken Beta actin

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD-ADH2t (17-21)

Plasmid

#101701

Purpose

ADH2t in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GCTT-[terminator of S. cerevisiae YMR303C (376 bp)]-CGCT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-PGK1 (01-11)

Plasmid

#101689

Purpose

pPGK1 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YCR012W (982bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST_TO_TAF15

Plasmid

#26378

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

TAF15 (TAF15 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCG-B-mSpyCatcher-C

Plasmid

#87374

Purpose

An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

mSpyCatcher

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCG-A-celA(SP)-B

Plasmid

#87371

Purpose

An ORF part-containing vector harbouring the signal peptide from CelA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

CelA signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCG-A-xynA(SP)-B

Plasmid

#87372

Purpose

An ORF part-containing vector harbouring the signal peptide from XynA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

XynA signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pFH42

Plasmid

#125881

Purpose

Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfPar3_4

Plasmid

#26706

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Par3 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Par3 RNAi

Available Since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only
pFH86

Plasmid

#128199

Purpose

improved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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