We narrowed to 11,977 results for: pcDNA

1. Nano-lantern(Ca2+)_600/pcDNA3

   Plasmid

   #51982

   Purpose

   mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM_E104Q-2G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

2. p4979 pcDNA4-E6AP III L502P

   Plasmid

   #32892

   Depositor

   Peter Howley

   Article

   Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

   Insert

   E6AP (UBE3A Human)
   

   Use

   Tags

   6xHis Express and T7-tag

   Expression

   Mammalian

   Mutation

   L502P

   Promoter

   CMV

   Available since

   May 13, 2013

   Availability

   Academic Institutions and Nonprofits only

3. p4978 pcDNA4-E6AP III E550L

   Plasmid

   #32891

   Depositor

   Peter Howley

   Article

   Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

   Insert

   E6AP (UBE3A Human)
   

   Use

   Tags

   6xHis Express and T7-tag

   Expression

   Mammalian

   Mutation

   E550L

   Promoter

   CMV

   Available since

   May 13, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA4-RI1-332-MYC-His6

   Plasmid

   #185351

   Purpose

   Mammalian expression of Ribophorin I

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   Ribophorin I (RPN1 Human)
   

   Use

   Tags

   Myc, 6xHis

   Expression

   Mammalian

   Mutation

   aa 1-332 only

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mouse PKA-Calpha-mPAGFP

   Plasmid

   #45524

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA catalytic subunit alpha tagged by mPAGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 FLIPglu-30uDelta13V Venus-Y67G

   Plasmid

   #18016

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   FLIPglu D154A Delta13V Venus-Y67G
   

   Use

   Tags

   ECFP and Venus-Y67G

   Expression

   Mammalian

   Mutation

   mglB-D154A; non-functional Venus

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p6042 pcDNA-Xpress-His-USP5

   Plasmid

   #24744

   Depositor

   Peter Howley

   Article

   Vos et al J Virol. 2009 Sep . 83(17):8885-92.

   Insert

   USP5 (USP5 Human)
   

   Use

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2010

   Availability

   Academic Institutions and Nonprofits only

8. EGFP Rab11a S25N pcDNA3.1 Zeo (+)

   Plasmid

   #206080

   Purpose

   dominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling

   Depositor

   Annette Dolphin

   Article

   Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

   Insert

   RAB11A (RAB11A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S25N

   Promoter

   CMV

   Available since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Cer(1-158)-beta-5 in pcDNAI/Amp

   Plasmid

   #55708

   Purpose

   An amino-terminal fragment of mCerulean was fused to Gbeta5. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

   Insert

   mCer(1-158)-beta5 (GNB5 Aequorea victoria, Human)
   

   Use

   Tags

   Cer(1-158) was fused to the amino terminus of Gbe…

   Expression

   Mammalian

   Mutation

   GBeta-5 was amplified via PCR, which added an N-t…

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA4-His-Max-A-PQBP1-Y65C

    Plasmid

    #25803

    Depositor

    Marius Sudol

    Article

    Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

    Insert

    PQBP1 with Y65C mutation (PQBP1 Human)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Tyrosine 65 within the WW domain of PQBP1 is chan…

    Promoter

    Available since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-N-mSOG-bFos-IRES-mCherry

    Plasmid

    #136599

    Purpose

    Encodes bFos domain tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

    Depositor

    John Ngo

    Article

    Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

    Insert

    N-mSOG-bFos
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p4973 pcDNA4-E6AP III C21Y

    Plasmid

    #32890

    Depositor

    Peter Howley

    Article

    Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

    Insert

    E6AP (UBE3A Human)
    

    Use

    Tags

    6 His Express and T7-tag

    Expression

    Mammalian

    Mutation

    C21Y

    Promoter

    CMV

    Available since

    May 13, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-RIPK3[S199A][S227A]-FLAG

    Plasmid

    #199323

    Purpose

    double mutated phosphorylation site (kinase acitivity and MLKL interaction inhibited)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 199 and 227 to Alanine

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 1121 pcDNA3 GFP FKHR H215R

    Plasmid

    #9027

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR H215R (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H215R

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1 6B CARD8 UPA-CARD-mCherry WT

    Plasmid

    #164011

    Purpose

    Mammalian expression vector encoding CARD8 UPA-CARD with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300

    Plasmid

    #204697

    Purpose

    This plasmid allows for inducible expression of engineered BRD4-p300 fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-p300 (BRD4, EP300 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    direct fusion of BRD4 to p300

    Promoter

    CMV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C261

    Plasmid

    #171429

    Purpose

    Expression of FKBP-(GGS)X6-HaloTagM C261 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    FKBP-(GGS)X6-HaloTag C261
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR

    Plasmid

    #123346

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-CKAR
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5 FRT TO-EGFP-G3BP1-S149A

    Plasmid

    #136004

    Purpose

    DOX-inducible S149A G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S149A

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5 FRT TO-EGFP-G3BP1-DeltaRBP

    Plasmid

    #136005

    Purpose

    DOX-inducible G3BP1 with RNA binding domains deleted inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C-term of G3BP1 Deleted for Delta RBP

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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