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pLG013 (DRT type 5)

Plasmid

#157891

Purpose

Expresses the DRT type 5 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 5

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-GFP-recipient

Plasmid

#11657

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling GFP expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pMS2

Plasmid

#191123

Purpose

Transcription of 12xMS2 repeats from a tetracycline-inducible promoter

Depositor

Ariel Lindner

Article

Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

Insert

MS2

Use

Synthetic Biology

Promoter

tetracycline-inducible promoter

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK-rABE-Flag

Plasmid

#191383

Purpose

Doxycycline inducible expression of RNA A-to-I editor rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

rABE-Flag

Use

Lentiviral and Synthetic Biology

Tags

Flag

Expression

Mammalian

Promoter

tet-inducible promoter

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-teLuc c-myc

Plasmid

#100026

Purpose

Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

teLuc, a NanoLuc mutant

Tags

c-myc

Expression

Mammalian

Mutation

D19S, D85N, C164H

Promoter

CMV

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pSB_init-Sb44

Plasmid

#159421

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb44

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb44

Tags

c-Myc and poly-His

Expression

Bacterial

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pWJ66

Plasmid

#46570

Purpose

Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9-w
CRISPR array

Use

CRISPR

Tags

rpoZ

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pACYC-prk:nptII

Plasmid

#239580

Purpose

Selection plasmid for Rubisco directed evolution

Depositor

Matthew D. Shoulders

Article

McDonald et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

PRK-NPTII

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLBH545_Tet-Cas14a1_Locus

Plasmid

#112501

Purpose

Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLP1

Plasmid

#209988

Purpose

Entry vector to store Level 0 parts

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLBH559_Tet-HisCas14a1Locus

Plasmid

#112502

Purpose

Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

6xHis-TEV

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly

Plasmid

#71499

Purpose

flySTARR-seq screening vector with DSCP core promoter.

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAFi-dHdrA

Plasmid

#184907

Purpose

carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cm-sgRNA-dCas9

Use

CRISPR

Expression

Bacterial

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-Tn7-Pcym

Plasmid

#225280

Purpose

Mini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.

Depositor

Mark Schembri

Article

Chang et al Appl Environ Microbiol. 2024 Oct 23;90(1

Type

Empty backbone

Expression

Bacterial

Promoter

Pcym cumate (p-isopropylbenzoate)-inducible promo…

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pKm-HCBE

Plasmid

#170333

Purpose

a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized CBEs

Use

CRISPR

Mutation

Cas9n-D10A

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMSP1E1D1

Plasmid

#161605

Purpose

Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E1D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGBT-RP2-1

Plasmid

#31828

Depositor

Stephen Ekker

Article

Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

Type

Empty backbone

Use

Cre/Lox

Tags

GFP GM2 variant and mRFP

Promoter

beta Actin

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pRK792

Plasmid

#8830

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219P mutant

Tags

His, MBP (cleaved), and polyarginine

Expression

Bacterial

Mutation

S219P mutation

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pMito-PAGFP-FRB.

Plasmid

#60005

Purpose

Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Type

Empty backbone

Use

Knocksideways

Tags

FRB and PAGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pPPAT-EGFP

Plasmid

#99105

Purpose

Expresses human PPAT-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

PPAT (PPAT Human)

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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