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pY001 (pFnCpf1_full)

Plasmid

#69973

Purpose

Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAV5S-F30-2xdBroccoli

Plasmid

#66845

Purpose

Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

Depositor

Samie Jaffrey

Article

Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Tags

5S rRNA

Expression

Mammalian

Promoter

5S

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

Plasmid

#61762

Purpose

Dual Color betaGlobin reporter to study splicing kinetics in vivo

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

b-Globin

Tags

24 X MS2 hairpin cassette in 3'UTR of b-Glob…

Expression

Mammalian

Promoter

Dox inducible Tet-ON promoter

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pDisplay-pHuji

Plasmid

#61556

Purpose

Red fluorescent protein based pH indicator in pDisplay vector

Depositor

Robert Campbell

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

pHuji

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pGFP FAK

Plasmid

#50515

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

Insert

protein tyrosine kinase

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau AP

Plasmid

#46905

Purpose

expresses EGFP tagged Tau AP in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau AP (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

all 14 S/P or T/P amino acid residues (T111, T153…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pQC membrane TdTomato IX

Plasmid

#37351

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

membrane TdTomato

Tags

Palmitoylation signal*

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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Myc-PSF-WT

Plasmid

#35183

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pLKO-puro FLAG SREBP1

Plasmid

#32017

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

SREBP1 (Srebf1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

V225M

Promoter

CMV

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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OCT4-eGFP-PGK-Puro

Plasmid

#31937

Purpose

Oct4 targeting donor vector

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Insert

OCT4 (POU5F1 Human)

Use

Targeting donor

Tags

eGFP

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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