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pAAV-hSyn-LMO7 (mNeonGreen-eKL9h-VChR1)

Plasmid

#205097

Purpose

fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Publication

Björefeldt et al Neurophotonics. 2024 Apr;11(2):021005. d

Insert

mNeonGreen, eKaz luciferase, Volvox Channelrhodopsin 1

Use

AAV

Promoter

hSyn

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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MSCVpuro-6xHis_SUMO2

Plasmid

#164938

Purpose

Expresses 6xHis tagged human Small Ubiquitin Like Modifier 2 (SUMO2) wild type cDNA

Depositor

Ji Luo

Publication

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Small Ubiquitin Like Modifier 2 (SUMO2 Human)

Use

Retroviral

Tags

6XHIS

Promoter

PGK promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC6-METAP1-3xFLAG

Plasmid

#163453

Purpose

lentiviral overexpression vector for human METAP1

Depositor

Jason Cantor

Publication

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

METAP1 (METAP1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available since

March 4, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescript II KS(+) ACMV DNA-A 1.4mer

Plasmid

#159134

Purpose

Infectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-A

Depositor

Vincent Fondong

Publication

Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

Insert

ACMV DNA-A 1.4mer

Use

Infectious clone for bombarding plants

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pNM3814

Plasmid

#154122

Purpose

mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::rtetR-L-QFAD::act-4 '3

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF10-2xCSTREP

Plasmid

#136174

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF10

Depositor

Britt Glaunsinger

Publication

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF10

Expression

Mammalian

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

Plasmid

#135812

Purpose

This plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

Depositor

Nadia Mercader Huber

Publication

Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

Insert

TagRFP

Use

Zebrafish

Promoter

UAS

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF45-2xCSTREP

Plasmid

#136206

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF45

Depositor

Britt Glaunsinger

Publication

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF45

Expression

Mammalian

Promoter

CMV

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc18a2

Plasmid

#124849

Purpose

Mutagenesis of Slc18a2

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc18a2 (Slc18a2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe(puro) mouse Egr3

Plasmid

#108102

Purpose

Retroviral vector for expression of mouse Egr3

Depositor

Peter Johnson

Publication

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr3 (Egr3 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+ mWnt5a

Plasmid

#102860

Purpose

Expresses mouse Wnt5a in mammalian cells, zebrafish or Xenopus

Depositor

Henry Ho

Publication

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mouse Wnt5a

Use

Zebrafish expression; xenopus expression

Expression

Mammalian

Promoter

CMV; SP6

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RAB10_pLX307

Plasmid

#98365

Purpose

Lentiviral expression of RAB10

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(T31N)-HA

Plasmid

#79429

Purpose

Expresses C-terminally HA-tagged Arf5(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Publication

Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

Insert

Arf5 (ARF5 Human)

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(Q71L)-HA

Plasmid

#79428

Purpose

Expresses C-terminally HA-tagged Arf5(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Publication

Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

Insert

Arf5 (ARF5 Human)

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 71 to Leucine

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-2Aneo

Plasmid

#80032

Purpose

Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

Depositor

Hiroyuki Konishi

Publication

Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

Type

Empty backbone

Use

AAV and Cre/Lox; A platform vector to construct t…

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB2

Plasmid

#63103

Purpose

Expression of HSPB2 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Publication

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hspb2 (HSPB2 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-Map7emtb

Plasmid

#46078

Depositor

Edgar Gomes

Publication

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Map7emtb

Tags

GFP

Expression

Mammalian

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pQTEV-TPT1

Plasmid

#31558

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

tumor protein, translationally-controlled 1 (TPT1 Human)

Tags

His and TEV

Expression

Bacterial

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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KNS2

Plasmid

#28122

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

KNS2:HPC09H-C05:C207000 (KLC1 Human)

Tags

His tag with TEV site

Expression

Bacterial

Available since

March 22, 2011

Availability

Industry, Academic Institutions, and Nonprofits
pXN-FBLWLF-GAL4DBD

Plasmid

#26264

Depositor

Tom Clandinin

Publication

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

ZIP- Gal4DBD (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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