We narrowed to 14,438 results for: NTS

1. pAtCas9-D10A_2

   Plasmid

   #91160

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_D10A (nickase)
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET42b-A3A-PBE-ΔUGI

   Plasmid

   #119771

   Purpose

   To express the recombinant APOBEC3A in vitro

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-NLS
   

   Tags

   6*HIS and NLS

   Expression

   Bacterial

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   T7 promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISP_IS

   Plasmid

   #120425

   Purpose

   This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

   Depositor

   Tamás Fehér

   Article

   Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

   Insert

   CRISPR spacers targeting IS1, IS5, IS3
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   constitutive

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPS0384

   Plasmid

   #133234

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   April 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPS0253

   Plasmid

   #115505

   Purpose

   Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ

   Plasmid

   #192380

   Purpose

   To test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p35S::mCh-HA-P2A-lifeact-mCh

   Plasmid

   #135234

   Purpose

   constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

   Depositor

   Armin Djamei

   Article

   Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

   Insert

   mCh-HA-P2A-lifeact-mCh
   

   Tags

   HA, lifeact, and p2a

   Expression

   Plant

   Promoter

   35S

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. p414GPD-3xFLAG-Nop4 WT

   Plasmid

   #169261

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WT

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Nop4 WT (NOP4 Budding Yeast)
   

   Tags

   3xFLAG

   Expression

   Yeast

   Promoter

   GPD

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. RnBarr2-mTurquoise2

   Plasmid

   #137792

   Purpose

   Visualization of beta-arrestin2

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat beta-arrestin2 (Arrb2 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD_A0801

    Plasmid

    #91022

    Purpose

    Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Mutation

    D10A, H840A

    Promoter

    35S

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGE671

    Plasmid

    #153237

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    GFP

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDECKO_Malat1_D

    Plasmid

    #72623

    Purpose

    Expresses two gRNAs targeting MALAT1 promoter

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward Malat1
    

    Expression

    Mammalian

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. EYFP-Clathrin-19

    Plasmid

    #56584

    Purpose

    Localization: Clathrin Vesicles, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Clathrin (CLTB Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pCEP4-myc-H2-Ld

    Plasmid

    #135519

    Purpose

    Mammalian expression of myc-tagged H2-Ld

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Ld
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGWB527

    Plasmid

    #74869

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. KHBD00322

    Plasmid

    #34447

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8591 (CTCF Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pMOD_A1610

    Plasmid

    #91038

    Purpose

    Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_D10A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-TaCas9_D10A nickase
    

    Use

    CRISPR

    Mutation

    D10A

    Promoter

    ZmUbi

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGWB525

    Plasmid

    #74867

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. KHBD00311

    Plasmid

    #34436

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG9786 (hb Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pJBL704

    Plasmid

    #140374

    Purpose

    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-4BP-tetO-3WJdB-T
    

    Use

    Synthetic Biology

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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