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We narrowed to 7,040 results for: SIM

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Showing: 6061 - 6080 of 7040 results

  3. pT2iApowriter2(#336)

    Plasmid
    #184104
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis
    Tags
    internal tag: 5myc
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184104-plasmid-map-sequence-id-364017

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  4. pT22Apowriter2(#340)

    Plasmid
    #184105
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    internal tag: 5myc
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184105-plasmid-map-sequence-id-363997

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  5. pT22iuasvApoWtEos3N(#486)

    Plasmid
    #184107
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    4xUAS
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184107-plasmid-map-sequence-id-363989

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  7. pT22iuasvApoWChe(#488)

    Plasmid
    #184108
    Purpose
    cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    4xUAS
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184108-plasmid-map-sequence-id-363970

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  8. pTW5126

    Plasmid
    #129732
    Purpose
    polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein
    Depositor
    James Fleckenstein
    Article
    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do
    Insert
    eatA-DUF::His8
    Tags
    9His tag
    Expression
    Bacterial
    Mutation
    replaces the domain of unknown function correspon…
    Promoter
    araBAD
    Available Since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    129732-plasmid-map-sequence-id-257612

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  10. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P90C

    Plasmid
    #114606
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 42 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Available Since
    Oct. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    114606-plasmid-map-sequence-id-225647

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  11. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P80C

    Plasmid
    #114602
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P80C cysteine mutations and a 22 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Available Since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114602-plasmid-map-sequence-id-224743

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  12. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-A60C

    Plasmid
    #114601
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 22 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, A…
    Available Since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114601-plasmid-map-sequence-id-222795

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  13. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P70C

    Plasmid
    #114605
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P70C cysteine mutations and a 42 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Available Since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114605-plasmid-map-sequence-id-222758

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  14. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-A60C

    Plasmid
    #114604
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 42 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, A…
    Available Since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114604-plasmid-map-sequence-id-222736

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  15. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P90C

    Plasmid
    #114603
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 22 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Available Since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114603-plasmid-map-sequence-id-222733

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  19. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid
    #124536
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1
    Inserts
    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    Use
    Luciferase and Synthetic Biology
    Expression
    Mammalian
    Available Since
    June 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    124536-plasmid-map-sequence-id-272178

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  20. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Human, Synthetic)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

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Showing: 6061 - 6080 of 7040 results