We narrowed to 7,531 results for: Ski;

1. pQCXIZ GFP PODXL 4K>R

   Plasmid

   #202420

   Purpose

   Expression of ubiquitination-deficient GFP-tagged PODXL (4 cytoplasmic lysines mutated to arginine)

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Mutation

   K431/525/526/547R

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_AKR1B1_WT

   Plasmid

   #82928

   Purpose

   Gateway Donor vector containing AKR1B1, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   AKR1B1 (AKR1B1 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV hSyn mCh-IRES-SspB(micro)-BoNT/B(147-441, Y365A)

   Plasmid

   #122986

   Purpose

   AAV plasmid with human synapsin promoter driving mCh and SspB(micro)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT

   Depositor

   Matthew Kennedy

   Article

   Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

   Insert

   mCh-IRES-SspB(micro)-BoNT/B(147-441)
   

   Use

   AAV

   Tags

   mCh

   Expression

   Mammalian

   Mutation

   SspB contains R73Q "micro" mutation, Bo…

   Promoter

   human synapsin

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1 _3xHA-Ago2_CATmut

   Plasmid

   #73540

   Purpose

   Inducible lentiviral expression of Ago2_CATmut

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   Ago2 (Ago2 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Catalytic mutant PIWI domain D670A

   Promoter

   TRE promoter, Tet ON

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK1520-LRRK2-G2019S-ng

   Plasmid

   #180436

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of LRRK2-G2019S mutation in PE3 approach

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-pegRNA-SNCA-A53T

   Plasmid

   #181738

   Purpose

   Transiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing pegRNA for SNCA-A53T (SNCA Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBPK1520-SNCA-A53T-ng

   Plasmid

   #181739

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approach

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing ngRNA for SNCA-A53T (SNCA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139097

   Purpose

   Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dxCas9-NG
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBPK1520-HEK3-CTTins-ng

   Plasmid

   #180019

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of HEK3 CTT insertion in PE3 approach

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing ngRNA for HEK3-CTTins (EPHA8 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Human U7

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCerulean-PCNA-PuroR

    Plasmid

    #215063

    Purpose

    Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PCNA (PCNA Human)
    

    Use

    Retroviral

    Tags

    mCerulean

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.ZAC.GFAP.iAChSnFR

    Plasmid

    #137962

    Purpose

    Expresses iAChSnFR under GFAP promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Insert

    iAChSnFR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    GFAP

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX RASGAP(N)-SH2

    Plasmid

    #46432

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Gap/RASGAP(N) (RASA1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 168-283

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. Fgf4enhLV (FpG12)

    Plasmid

    #69444

    Purpose

    positive control plasmid for lentiviral reporter expression in ESCs

    Depositor

    Lisa Dailey

    Article

    Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

    Inserts

    minimal FGF4 promoter
    EGFP
    Ubiquitin promoter
    Hygromycin resistance
    FGF4 enhancer (Fgf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_PCCB_WT_V5

    Plasmid

    #82938

    Purpose

    Gateway Donor vector containing PCCB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    PCCB (PCCB Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid

    #106183

    Purpose

    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-Venus-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

    Promoter

    hSynapsin

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIBN-tagBFP-hTRF1

    Plasmid

    #103801

    Purpose

    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-hTRF1 (CIB1, TERF1 Human, Mustard Weed)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. mKO2-CDK4

    Plasmid

    #215065

    Purpose

    Expresses mKO2-CDK4 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Retroviral

    Tags

    mKO2

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

    Plasmid

    #169336

    Purpose

    Expression of the LT-Fucci(SA) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Fucci(SA) (GMNN, CDT1 Human)
    

    Tags

    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_SMAD4_p.P356R

    Plasmid

    #82769

    Purpose

    Gateway Donor vector containing SMAD4, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    SMAD4 (SMAD4 Human)
    

    Use

    Gateway entry vector

    Mutation

    P356R

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-pegRNA-SNCA-A30P

    Plasmid

    #180016

    Purpose

    Transiently expressing a pegRNA to introduce SNCA-A30P mutation in human cells

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    Prime editing pegRNA for SNCA-A30P (SNCA Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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