We narrowed to 6,946 results for: crispr cas9 plasmids

1. pU6-1 (GB1204)

   Plasmid

   #75405

   Purpose

   Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   AtU6-1 promoter
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_FLAG_hSF3B1

   Plasmid

   #176590

   Purpose

   Adenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1

   Depositor

   Manoj Pillai

   Article

   Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

   Insert

   SF3B1 (SF3B1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_NPM2_cterm1

   Plasmid

   #222911

   Purpose

   Cas9/sgRNA plasmid for targeting NPM2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NPM2 sgRNA 1 (NPM2 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330_NPM2_cterm4

   Plasmid

   #222914

   Purpose

   Cas9/sgRNA plasmid for targeting NPM2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NPM2 sgRNA 4 (NPM2 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330_NPM2_cterm3

   Plasmid

   #222913

   Purpose

   Cas9/sgRNA plasmid for targeting NPM2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NPM2 sgRNA 3 (NPM2 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_NPM2_cterm2

   Plasmid

   #222912

   Purpose

   Cas9/sgRNA plasmid for targeting NPM2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NPM2 sgRNA 2 (NPM2 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PEA1-Puro-HEK3 CTT ins

   Plasmid

   #171996

   Purpose

   Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PEA1-Nuclease-Puro HEK3 CTT ins

   Plasmid

   #171995

   Purpose

   Delivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

   Plasmid

   #217306

   Purpose

   sgRNA mcherry + Puromycin

   Depositor

   Luke Gilbert

   Insert

   pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6, Ef1alpha

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP)

    Plasmid

    #217305

    Purpose

    sgRNA BFP + Puromycin

    Depositor

    Luke Gilbert

    Insert

    pU6-sgRNA, Ef1alpha-Puro-T2A-BFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    U6, Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60225

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid

    #60230

    Purpose

    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    EGFP
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA-P2A and EGFP-KASH

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hSyn

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGmCherry-gRNA

    Plasmid

    #87110

    Purpose

    gRNA cloning vector with CAGmCherry

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

    Plasmid

    #113158

    Purpose

    An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

    Plasmid

    #113156

    Purpose

    An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat Rosa26
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOTTC1079 - pAAV TH gRNA A EF1a EGFP

    Plasmid

    #113159

    Purpose

    An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

    Plasmid

    #113155

    Purpose

    An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

    Plasmid

    #113157

    Purpose

    An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat MANF
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUCmini-UGCG-tdTomato

    Plasmid

    #225879

    Purpose

    Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertion

    Depositor

    Yohei Sato

    Article

    Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

    Insert

    tdTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUCmini-UGCG-EGFP

    Plasmid

    #225878

    Purpose

    Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion

    Depositor

    Yohei Sato

    Article

    Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge