We narrowed to 9,085 results for: mel

1. pLVX-anti-MIR328-3p

   Plasmid

   #153319

   Purpose

   Expresses zsGreen fused to a miR-328-3p sponge and mCherry in mammalian cells.

   Depositor

   Robert Judson-Torres

   Article

   McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

   Insert

   zsGreen with MIR328-3p sponge
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-anti-MIR211-5p

   Plasmid

   #153318

   Purpose

   Expresses zsGreen fused to a miR-211-5p sponge and mCherry in mammalian cells.

   Depositor

   Robert Judson-Torres

   Article

   McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

   Insert

   zsGreen with MIR211-5p sponge
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSJ982

   Plasmid

   #176622

   Purpose

   Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

   Depositor

   Alexander Varshavsky

   Article

   Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

   Insert

   Ub-MPGLW-Fbp1
   

   Tags

   Ubiquitin N-terminal fusion and triple flag tag C…

   Expression

   Yeast

   Mutation

   Changed first 4 N-terminal amino acid residues of…

   Promoter

   TDH3tc3

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIN-Gapdh

   Plasmid

   #175938

   Purpose

   knockout mouse Gapdh

   Depositor

   Dan Littman

   Article

   Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

   Insert

   Gapdh gRNA (Gapdh Mouse)
   

   Use

   Retroviral

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-3xFLAG-Puro PAK5 K478R/479R S364L

   Plasmid

   #167199

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R S364L; PAK7 Kinase dead & S364L double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R S364L
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGG198

   Plasmid

   #165605

   Purpose

   Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFP

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Secondary protospacer ('CGGCG' PAM) ins…

   Promoter

   lac

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-3xFLAG-Puro PAK5 K478R/479R D421N

   Plasmid

   #167200

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R D421N
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-3xFLAG-Puro PAK5 K478R/479R E294K

   Plasmid

   #167198

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R E294K
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-3xFLAG-Puro PAK5 K478R/479R M173I

   Plasmid

   #167197

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R M173I; PAK7 Kinase dead & M173I double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R M173I
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-3xFLAG-Puro PAK5 K478R/479R E144K

    Plasmid

    #167196

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E144K; PAK7 Kinase dead & E144K double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R E144K
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGG438

    Plasmid

    #165486

    Purpose

    Vector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAG

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    Mammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    D1135V, S1136R, D1332K, and R1333G mutations in S…

    Promoter

    CMV and T7

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. INCTbiosyn-pUB-HspINT4

    Plasmid

    #127513

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 4.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 4 coding sequence codon optimized for H. sapiens expression.
    

    Expression

    Mammalian

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. INCTbiosyn-pUB-HspINT5

    Plasmid

    #127514

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 5.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 5 coding sequence codon optimized for H. sapiens expression.
    

    Expression

    Mammalian

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBADHISTEV_PfeEH258A

    Plasmid

    #129122

    Purpose

    expresses H258A mutant of PfeE in E.coli

    Depositor

    James Naismith

    Article

    Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

    Insert

    Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
    

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed, mutat…

    Promoter

    PBAD

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBADHISTEV_PfeES157A

    Plasmid

    #129121

    Purpose

    expresses S157A mutant of PfeE in E.coli

    Depositor

    James Naismith

    Article

    Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

    Insert

    Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
    

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed, mutat…

    Promoter

    PBAD

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Designed HEL-binding scFv D44.1des

    Plasmid

    #111719

    Purpose

    Yeast Surface Display of D44.1des

    Depositor

    Sarel Fleishman

    Article

    Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

    Insert

    D44.1 des
    

    Tags

    cmyc tag

    Expression

    Yeast

    Mutation

    L Q89G, L S55V, L S43P, H V37M, H H43G, H W47Y, H…

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Dscam1_7.27.26 EC10-Fc-His

    Plasmid

    #72189

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.26
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dscam1_7.27.23 EC10-Fc-His

    Plasmid

    #72187

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.23
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Dscam1_7.27.26 EC10-AP-His

    Plasmid

    #72063

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.26
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GAL-ARF

    Plasmid

    #89121

    Purpose

    mammalian expression of p14-hARF linked to GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

    Insert

    p14-hARF (CDKN2A Human)
    

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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