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NmCAT

Plasmid

#241909

Purpose

Heterologous expression of 4-hydroxybutyrate-CoA synthetase from Nitrosopumilis maritimus

Depositor

Neil Dixon

Article

Zheng et al Biochem. Eng. J. 2024 Sep 16; 212. doi:

Insert

4HB-CoA synthetase

Expression

Bacterial

Mutation

Mutated Haloferax gyrase B (gyrB*)

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHR Ef1a_mAbCII-synNotch_tTA-PGK_Puro

Plasmid

#223538

Purpose

Anti-collagen type II scFv-based synNotch; puro-selectable

Depositor

Jonathan Brunger

Article

Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

Insert

mAbCII_synNotch-tTA

Use

Lentiviral

Tags

c-myc tag

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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PKT2/CLP-Vector

Plasmid

#239611

Purpose

Transposon vector

Depositor

Xin-yuan Guan

Article

Gong et al Cell. 2025 Jul 24:S0092-8674(25)00793-7.

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHR TRE_firefly Luciferase_2A_mCherry-PGK_BFP

Plasmid

#223539

Purpose

Inducible firefly luciferase and mCherry; constitutive BFP

Depositor

Jonathan Brunger

Article

Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

Inserts

Enhanced firefly luciferase
mCherry
TagBFP

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

June 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHR Ef1a_LaG16-synNotch_tTA-PGK_Puro

Plasmid

#223536

Purpose

Anti-GFP LaG16 nanobody-based synNotch; puro-selectable

Depositor

Jonathan Brunger

Article

Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

Insert

LaG16_synNotch_tTA

Use

Lentiviral

Tags

c-myc tag

Expression

Mammalian

Available Since

June 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHR TRE_SEAP_IRES_mCherry-PGK_BFP-hf

Plasmid

#223535

Purpose

Inducible secreted embryonic alkaline phosphatase and mCherry; constitutive BFP

Depositor

Jonathan Brunger

Article

Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

Inserts

Secreted alkaline phosphatase
mCherry
TagBFP

Use

Lentiviral

Available Since

June 17, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-BFP

Plasmid

#227505

Purpose

Backbone for CARGO cloning, with U6 promoter and gRNA scaffold, TagBFP, and Kanamycin resistence

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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YH039 PGK-TO-FKBP-tm-GS-XTEN-ADAR2(DD-E488Q-T501A)

Plasmid

#233378

Purpose

PGK promoter driven FKBP fused with ADAR2dd (E488Q,T501A), with a CD28 transmembrane domain in between

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

PGK

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F

Plasmid

#200877

Purpose

ZooTsy_TnsF expression construct for protein purification.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy_TnsF

Expression

Bacterial

Mutation

TnsF-Y584F

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT384: pSC101-Donor(ZooTsy)

Plasmid

#200875

Purpose

ZooTsy donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT81: pTarget(ZooTsy)

Plasmid

#200876

Purpose

Target plasmid containing attachement site for ZooTsy.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy_comM

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT315: pHelper(ZooTsy)_TnsF-Y584F

Plasmid

#200872

Purpose

Inducible expression of ZooTsy proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Mutation

TnsF-Y584F

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT375: pDonor(ZooTsy)_excision-reporter

Plasmid

#200873

Purpose

Reporter for ZooTsy excision.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT155: pHelper(ZooTsy)_ΔHTH

Plasmid

#200868

Purpose

Inducible expression of ZooTsy (except HTH) proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT156: pHelper(ZooTsy)_ΔTnsF

Plasmid

#200869

Purpose

Inducible expression of ZooTsy (except TnsF) proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT148: pHelper(ZooTsy)

Plasmid

#200866

Purpose

Inducible expression of ZooTsy proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT154: pHelper(ZooTsy)_ΔYRec

Plasmid

#200867

Purpose

Inducible expression of ZooTsy (except YRec) proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT368: pSC101-Donor(AjTn6022)

Plasmid

#200864

Purpose

AjTn6022 donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT112: pTarget(AjTn6022)_U50D50

Plasmid

#200862

Purpose

Target plasmid containing attachement site for AjTn6022.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022_comM

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT91: pR6K-Donor(AjTn6022)

Plasmid

#200863

Purpose

AjTn6022 donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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