We narrowed to 10,400 results for: plasmids 101

1. pDuRCC-K

   Plasmid

   #81193

   Purpose

   Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below., Please view…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAT003

   Plasmid

   #171628

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-TRAC
   mNeon
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CAG and EF1-a

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJBEI-6411

   Plasmid

   #47050

   Purpose

   BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli

   Depositor

   Taek Soon Lee

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Insert

   ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
   

   Use

   Synthetic Biology; Bglbrick

   Expression

   Bacterial

   Promoter

   Pbad

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P-1-eIF4E K119A

   Plasmid

   #199440

   Purpose

   Bacterial expression plasmids for production of recombinant GST-eIF4E K119A

   Depositor

   Shin-ichi Hoshino

   Article

   Ogami et al STAR Protoc. 2023 May 26;4(2):102340. do

   Insert

   1977 (EIF4E Human)
   

   Tags

   GST-fusion protein

   Expression

   Bacterial

   Mutation

   changed lysine 119 to alanine

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Bxb1-GA_AIO-TetOn_mScarlet_Down-Tandem

   Plasmid

   #229792

   Purpose

   Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   TRE and CAG

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1000.pCCLsin.cPPT.hPGK.Brn2.WPRE

   Plasmid

   #67294

   Purpose

   Induce human iN conversion from fibroblast

   Depositor

   Malin Parmar

   Article

   Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

   Insert

   Brn2 (Pou3f2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. IsHSP70-mCherry

   Plasmid

   #220092

   Purpose

   Expresses in ISE6 (Ixodes scapularis) cells, heat inducible. Plasmid backbone derived from pGL-4.79 [Rluc] (Promega)

   Depositor

   Monika Gulia-Nuss

   Article

   Pham et al iScience. 2024 Jul 8;27(8):110468. doi:

   Insert

   HSP70 promoter from Ixodes scapularis
   

   Promoter

   IsHSP70

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. eGFPbait-E2A-KalTA4-pA donor vector

   Plasmid

   #61069

   Purpose

   for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)

   Depositor

   Filippo Del Bene

   Article

   Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

   Inserts

   eGFPbait
   KalTA4
   

   Use

   Zebrafish expression

   Tags

   E2A

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3xFlag-HaloTag-SUZ12 HDR - pDY082

   Plasmid

   #171110

   Purpose

   Homology directed repair plasmid for generating HaloTag-SUZ12 cells

   Depositor

   Thomas Cech

   Article

   Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

   Insert

   SUZ12 (SUZ12 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQCXIP-GFP-LmnB1

    Plasmid

    #164249

    Purpose

    retroviral plasmid for GFP-LmnB1

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    LMNB1 (LMNB1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-pMecp2-SaCas9-pU6-sgRNA

    Plasmid

    #159173

    Purpose

    Vector A encodes pAAV-pMecp2-SaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of SaCas9-sgRNA in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-pMecp2-dSaCas9-VP160-pU6-sgRNA

    Plasmid

    #158987

    Purpose

    Vector D encodes pAAV-pMecp2-dSaCas9-VP160-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP160
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEW3: pET-32a Dot1L(2-416)

    Plasmid

    #124098

    Purpose

    Dot1L(2-416) expression plasmid

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

    Insert

    Dot1L(2-416) (DOT1L Human)
    

    Tags

    Trx-His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Syn-FLEX-ExSYTE

    Plasmid

    #216263

    Purpose

    Cre-dependent expressoin of ExSYTE (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoter

    Depositor

    Susumu Tomita

    Article

    Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

    Insert

    ExSYTE P2A EGFP - Rat DTE
    

    Use

    AAV

    Available Since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-fDIO-mRuby3-2A-dCre

    Plasmid

    #203845

    Purpose

    Flp-dependent enzymatically inactive Cre and mRuby3 expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    dCre
    

    Use

    AAV

    Tags

    mRuby3-2A

    Mutation

    Enzymatically inactive Cre

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-pMecp2-dSaCas9-pU6-sgRNA

    Plasmid

    #159174

    Purpose

    Vector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. (326) pAAV SV40-ZsGreen U6-gRNA

    Plasmid

    #163020

    Purpose

    Fluorescent reporter for Sa gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Zs Green
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

    Plasmid

    #221080

    Purpose

    SEMPER plasmid for expression of gas vesicles using mammalian acoustic reporter genes (mARGs)

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRiboI-APEX2m

    Plasmid

    #176843

    Purpose

    Expression of APEX2 from a codon-optimized gene (single-copy integrating plasmid)

    Depositor

    Jessica Seeliger

    Article

    Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

    Insert

    pRibo-APEX2m
    

    Use

    Integrating

    Expression

    Bacterial

    Promoter

    pRibo, theophilline riboswitch inducible hsp60 pr…

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-HCMVgL-FLAG (Merlin)

    Plasmid

    #136450

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein L (UL115 Human betaherpesvirus 5)
    

    Tags

    Short SGGSG linker and FLAG tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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