We narrowed to 11,681 results for: cel.2

1. PCBP4_3xKH_pGEX

   Plasmid

   #135156

   Purpose

   Recombinant expression of PCBP4 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   PCBP4 (PCBP4 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. JARID2-KO-gRNA-1

   Plasmid

   #131328

   Purpose

   gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   JARID2-KO-gRNA-1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

3. EED-KO-gRNA-1

   Plasmid

   #131321

   Purpose

   gRNA to knockout EED. Use with EED-KO-gRNA-2

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EED-KO-gRNA-1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

4. RBMS2_2xRRM_pGEX

   Plasmid

   #135132

   Purpose

   Recombinant expression of RBMS2 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   RBMS2 (RBMS2 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. HsTPC2-EYFP

   Plasmid

   #135194

   Purpose

   Expresses tagged TPC2 in mammalian cells

   Depositor

   Antony Galione

   Article

   Davis et al Curr Biol. 2012 Dec 18;22(24):2331-7. do

   Insert

   TPC2 (TPCN2 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-NT-3/D15A

   Plasmid

   #73035

   Purpose

   Express an NT-3-BDNF hybrid neurotrophic factor in mammalian cells

   Depositor

   Pantelis Tsoulfas

   Article

   Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

   Insert

   NT-3/D15A (NTF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Mutated Aspartic acid in position 15 of the matur…

   Promoter

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. LifeAct-14 (LA-14)

   Plasmid

   #158750

   Purpose

   Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.

   Depositor

   Minhajuddin Sirajuddin

   Article

   Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

   Insert

   LifeAct-14
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Deletion mutation for last three amino acids from…

   Promoter

   CMV immediate early

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. tetO.Nfia.Blast

   Plasmid

   #117270

   Purpose

   Expresses Nfia and blasticidin resistance gene under control of tetO sequence.

   Depositor

   Henrik Ahlenius

   Article

   Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

   Insert

   Nfia (Nfia Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-GFP-WT1 +KTS N terminal HA

   Plasmid

   #217639

   Purpose

   Entry vector with WT1 +KTS mutant with N terminal HA for gateway cloning

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 +KTS isoform (WT1 Human)
   

   Use

   Gateway cloning

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-GFP-WT1 -KTS N terminal HA

    Plasmid

    #217641

    Purpose

    Entry vector with WT1 -KTS mutant with N terminal HA for gateway cloning

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 -KTS isoform (WT1 Human)
    

    Use

    Gateway cloning

    Tags

    HA

    Expression

    Mutation

    p.408_410delKTS

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lifeact-7-iRFP670

    Plasmid

    #103032

    Purpose

    Expression of iRFP670-tagged Lifeact with G418 selection in mammalian cells

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-Blast coSHMT2

    Plasmid

    #106299

    Purpose

    Expresses SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 2 (SHMT2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-OC-IRES-BSD

    Plasmid

    #53118

    Purpose

    To create stable cell clones with high-level expression of Cas9 and OCT1

    Depositor

    Wensheng Wei

    Article

    Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Inserts

    OCT1 (POU2F1 Human)
    Cas9
    

    Use

    Lentiviral

    Tags

    IRES-BSD, NLS, and P2A

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-mPCSK8

    Plasmid

    #122677

    Purpose

    Expresses mouse PCSK8 in mammalian cells

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-HCF-1rep1E10A

    Plasmid

    #84607

    Purpose

    Plasmid contains HCF-1rep1E10A with GST-tag

    Depositor

    Winship Herr

    Article

    Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

    Insert

    HCF-1rep1E10A (HCFC1 Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-HCF-1rep1wt

    Plasmid

    #84606

    Purpose

    Plasmid contains HCF-1rep1wt with GST-tag

    Depositor

    Winship Herr

    Article

    Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

    Insert

    HCF-1rep1wt (HCFC1 Human)
    

    Use

    Tags

    GST-TEV and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB-RTP2-Integration

    Plasmid

    #129459

    Purpose

    piggyBac transposon vector that Inducibly (Tet-On) expresses RTP2

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    RTP2 (RTP2 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV sMTase (eGFP)

    Plasmid

    #89930

    Purpose

    Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

    Depositor

    Carl Novina

    Article

    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

    Insert

    dCas9-MC-IRES-MN
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    deactivated Cas9

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKrox24(2xD-E)DsRed

    Plasmid

    #200111

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    modified EGR1 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    2 copies of D-E element, no other promoter elemen…

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. hs-lambda btl

    Plasmid

    #17581

    Depositor

    Denise Montell

    Article

    Lee et al Genes Dev. 1996 Nov 15. 10(22):2912-21.

    Insert

    lambda-btl (btl Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

21. pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)

    Plasmid

    #61943

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-156)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Residues 2-39 deleted resulting in construct star…

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pENTR-GFP-WT1 +KTS C terminal HA

    Plasmid

    #217640

    Purpose

    Entry vector with WT1 +KTS mutant with C terminal HA for gateway cloning

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 +KTS isoform (WT1 Human)
    

    Use

    Gateway cloning

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pENTR-GFP-WT1 -KTS C terminal HA

    Plasmid

    #217642

    Purpose

    Entry vector with WT1 -KTS mutant with C terminal HA for gateway cloning

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 -KTS isoform (WT1 Human)
    

    Use

    Gateway cloning

    Tags

    HA

    Expression

    Mutation

    p.408_410delKTS

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDEST-hMeCP2-GFP

    Plasmid

    #48078

    Purpose

    Mammalian expression of GFP tagged MeCP2-WT

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. FGFR1-optoDroplet

    Plasmid

    #111509

    Purpose

    For optogenetic clustering of FGFR1

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

    Insert

    FGFR1 (FGFR1 Human)
    

    Use

    Lentiviral

    Tags

    Cry2, FUS, Fusion Red, and Myristoylation sequenc…

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-Sox2-CreERT2

    Plasmid

    #135618

    Purpose

    Donor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    P2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    P2A-CreERT2

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-FLEX-SaCas9-U6-sgGabrg2

    Plasmid

    #124857

    Purpose

    Mutagenesis of Gabrg2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg2 (Gabrg2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKrox24(2xD-E)dTomato

    Plasmid

    #200110

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    modified EGR1 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    2 copies of D-E element, no other promoter elemen…

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pscAAV-Sox2-mStr

    Plasmid

    #135617

    Purpose

    Donor vector for genomic targeting of a P2A-mStrawberry cassette to the 3' end of the mouse Sox2 locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    P2A-mStrawberry flanked by Sox2 homology arms (Sox2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    P2A-mStrawberry

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ss-SNAP-TMD-NS3-Gal4

    Plasmid

    #112279

    Purpose

    Encodes the membrane-targeted TMD-NS3-Gal4, which exhibits drug-inducible transcription "turn-off" activity. SNAP-tag is fused to the extracellular region of the encoded protein construct.

    Depositor

    John Ngo

    Article

    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

    Insert

    SNAP-TMD-NS3-Gal4
    

    Use

    Tags

    AU1 (N term on NS3) and HA (C term on NS3)

    Expression

    Mammalian

    Mutation

    NS3 mutant T54A

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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