We narrowed to 8,600 results for: Set

1. pAG32-L-IFP-F[2] (clon 3)

   Plasmid

   #52900

   Purpose

   Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   L-IFP-F[2]
   

   Use

   Vector for homologous recombination

   Promoter

   TEF promoter

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

2. LentiGuide-BC-CMV-Puro

   Plasmid

   #127169

   Purpose

   PCR template for CMV-puro resistance cassette

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV TRE-GFP-TMD-DLL1

   Plasmid

   #194936

   Purpose

   LV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   eGFP-(PDGFR)TMD-rDLL1 ICD (WT)
   

   Use

   Lentiviral

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRES.Puro.EGFP

   Plasmid

   #24176

   Depositor

   Robert Sobol

   Article

   Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. do

   Insert

   EGFP
   

   Expression

   Mammalian

   Mutation

   x

   Available Since

   April 9, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pCfB8380

   Plasmid

   #126914

   Purpose

   Plasmid containing integration cassette at XII-5 integration site in Saccharomyces cerevisiae for overexpression of XdCrtE

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtE
   

   Expression

   Yeast

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. GGE038

   Plasmid

   #120785

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown_Zeta-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. GGE067

   Plasmid

   #120784

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp_Zeta-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ABCB8 (ABCB8A-c016)

   Plasmid

   #110266

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ABCB8 (ABCB8 Human)
   

   Tags

   TEV-His10-Flag

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. BASIC_7_J23101-RBS34-mCherry-B0015

   Plasmid

   #68141

   Purpose

   storage vector for BASIC part: RFP expression cassette

   Depositor

   Geoffrey Baldwin

   Article

   Storch et al ACS Synth Biol. 2015 Apr 16.

   Insert

   J23101-RBS34-mcherry-B0015
   

   Expression

   Bacterial

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAZ-donor

    Plasmid

    #112359

    Purpose

    CRISPR donor plasmid to tag human transcription factor MAZ with GFP

    Depositor

    Kevin White

    Insert

    MAZ homology arms flanking EGFP-IRES-Neo cassette (MAZ Human)
    

    Use

    CRISPR

    Mutation

    hg38:16:29809765:G>A

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Thy1.2-NLS_HA_Dre-P2A-BFP

    Plasmid

    #51275

    Purpose

    Co-expression of the site-specific recombinase Dre and BFP under the control of the Thy1.2 expression cassette

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS_HA_Dre-P2A-BFP
    

    Promoter

    Thy1.2

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenticrispr-wt-ltr-puro

    Plasmid

    #173428

    Purpose

    Expresses Cas9 and guide RNA, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiguide-puro-wt-ltr

    Plasmid

    #182635

    Purpose

    Expresses guide RNA, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. YN2_1_IL50_HOlocus

    Plasmid

    #194426

    Purpose

    Expresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

    Insert

    Cas9
    

    Expression

    Yeast

    Mutation

    NA

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid

    #69548

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICSL70002

    Plasmid

    #50333

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. MTK8b_005

    Plasmid

    #123893

    Purpose

    Encodes the 5' AAV ITR cassette as a type 8b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    5' AAV ITR
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3PA-Puro-iTk

    Plasmid

    #109067

    Purpose

    inserts three polyA sites to end transcription of a targetted gene and a puromycin cassette for selection

    Depositor

    Eric Lander

    Article

    Engreitz et al Nature. 2016 Nov 17;539(7629):452-455. d

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PV247

    Plasmid

    #132338

    Purpose

    CasA constitutive expression cassette for Zea mays

    Depositor

    Joshua Young

    Article

    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

    Insert

    CasA
    

    Expression

    Plant

    Mutation

    Codon optimize for expression and stability in Ze…

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTFE3-donor

    Plasmid

    #112377

    Purpose

    CRISPR donor plasmid to tag human transcription factor TFE3 with GFP

    Depositor

    Kevin White

    Insert

    TFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
    

    Use

    CRISPR

    Mutation

    hg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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