We narrowed to 40,052 results for: lat

1. pLL5.0-MRLC-AA-GFP

   Plasmid

   #187259

   Purpose

   Lentiviral expression of myosin regulatory light chain-AA mutant

   Depositor

   Alpha Yap

   Article

   Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Mutation

   T18A,S19A

   Promoter

   5' LTR

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. SMC2-mAC donor (Hygro)

   Plasmid

   #140648

   Purpose

   SMC2 tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   SMC2 (SMC2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK321 (TOP2A-mAC (Neo))

   Plasmid

   #140652

   Purpose

   TOP2A tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)

   Plasmid

   #140533

   Purpose

   OsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   mAID-EGFP-NLS TOL2
   

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSicoR-Ef1a-mCh

   Plasmid

   #31847

   Purpose

   Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   U6 for shRNA; Ef1alpha for mCherry

   Available Since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

   Plasmid

   #55200

   Purpose

   Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   dsRed2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI

   Plasmid

   #117005

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pinducer20-BSD-scFv-sfGFP-DNMT3A1

   Plasmid

   #115780

   Purpose

   targeted DNA methylation tool

   Depositor

   Margaret Goodell

   Article

   Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

   Insert

   scFv-sfGFP-DNMT3A1 (DNMT3A Human)
   

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-2T-GST-eIF4E K119A

   Plasmid

   #112818

   Purpose

   Expresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Eif4e (Eif4e Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   K119 mutated to A, increasing affinity for cap st…

   Promoter

   T7

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid

    #195741

    Purpose

    Base editing for making a pre-stop codon in mmoX using phenol inducible system

    Depositor

    Seung-Goo Lee

    Article

    Jeong et al Chem Eng J, 2023

    Insert

    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Po (phoenol-inducible promoter)

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1 p18 shRNA

    Plasmid

    #26631

    Depositor

    David Sabatini

    Article

    Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

    Insert

    p18 shRNA (LAMTOR1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

12. CRY2olig-mCh-CLC

    Plasmid

    #60035

    Purpose

    Expresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chain

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2-mCh-Clathrin Light Chain (CRY2 Mouse, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    M354I, E490G CRY2, A9D*

    Promoter

    CMV

    Available Since

    Oct. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-pro-sumo1

    Plasmid

    #25101

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO1 (SUMO1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Changed Serine 2 to Alanine

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

14. DHFR-mNeon-GluA1

    Plasmid

    #173009

    Purpose

    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    DHFR-mNeon-GluA1
    

    Tags

    DHFR and mNeon

    Expression

    Mammalian

    Promoter

    Chicken beta actin (CAG)

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHM151Env

    Plasmid

    #139017

    Purpose

    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

    Depositor

    Anjali Joshi

    Article

    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

    Insert

    HIV-1 Envelope Glycoprotein (env HIV-1)
    

    Expression

    Mammalian

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCD565

    Plasmid

    #153027

    Purpose

    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

    Depositor

    Jesse Zalatan

    Article

    Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

    Insert

    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV2-SynTetOff-FGL

    Plasmid

    #190234

    Purpose

    AAV vector, high-level expression of myrGFP with a somatodendritic-targeting signal (FGL) in neuronal cells

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    LDLR C-terminal sequence and myristoylation signa…

    Expression

    Mammalian

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PGK ARF6(Q67L)-CFP

    Plasmid

    #200428

    Purpose

    Mammalian expression of Q67L mutation in ARF6

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    ARF6 (ARF6 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    PGK

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV3Tag8li-hMBOAT7-3xFLAG

    Plasmid

    #165474

    Purpose

    Human MBOAT7 mammalian expression vector with 3 C-terminal FLAG tags.

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    Membrane bound O-acyltransferase domain containing 7 (MBOAT7 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. scFv-GCN4-DNMT3a-DNMT3l

    Plasmid

    #154140

    Purpose

    Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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