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pcDNA3.1-2xFLAG-2xSTREP (empty vector)

Plasmid

#172604

Purpose

Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Type

Empty backbone

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-neo

Plasmid

#166467

Purpose

Empty vector to express overexpression cDNA

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Type

Empty backbone

Use

Lentiviral

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-XBP1s

Plasmid

#63680

Purpose

Mammalian expression of Flag tagged XBP1s

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

spliced

Promoter

CMV

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAG109 H2B-FAST

Plasmid

#130722

Purpose

Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

Depositor

Arnaud Gautier

Article

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

H2B-FAST

Tags

myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-LMW-FGF2

Plasmid

#157663

Purpose

Expresses FGF2 in mammalian cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Tags

HA tag

Expression

Mammalian

Mutation

deleted amino acid 1-134

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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CPSF1

Plasmid

#155475

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPSF1 (CPSF1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pF CAG luc hygro

Plasmid

#67502

Purpose

Constitutive expression of firefly luciferase in mammalian cells

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Rab4a-C-7

Plasmid

#231781

Purpose

Visualization of endosome in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Rab4a-C-7 (RAB4A Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRVdGL-4Cre

Plasmid

#98039

Purpose

Second-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

mCre

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTRE3G-EBF1

Plasmid

#96965

Purpose

Dox-inducible expression of Ebf1 in mammalian cells

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

Ebf1 (Ebf1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-eFFly-eGFP

Plasmid

#104834

Purpose

eFFly luciferase for invivo Imaging, eGFP for flourescent detection

Depositor

Irmela Jeremias

Article

Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

Inserts

eFFly
eGFP

Use

Lentiviral and Luciferase; Egfp

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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IGF2BP1

Plasmid

#155676

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

IGF2BP1 (IGF2BP1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pShuttle-Flag-XBP1u

Plasmid

#63679

Purpose

Adenoviral shuttle vector for XBP1u expression

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

unspliced

Promoter

CMV

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131004

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA

Plasmid

#216319

Purpose

Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Cerulean + splice acceptor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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DMPK

Plasmid

#155550

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DMPK (DMPK Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)Luc2-Turbo RFP/CMV/WPREdU3

Plasmid

#136959

Purpose

Reporter vector encoding firefly luciferase and TurboRFP fluorescent protein

Depositor

Jakob Reiser

Article

Gills et al Oncotarget. 2018 Aug 24;9(66):32718-3272

Insert

Firefly luciferase and TurboRFP

Use

Lentiviral

Tags

V5 tag

Promoter

CMV

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5AdeltaSIV GFP Nter

Plasmid

#145770

Purpose

expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

Depositor

Hugues Abriel

Article

Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Tags

GFP

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFB-GST-FBXO22

Plasmid

#228367

Purpose

express human FBXO22 in insect cells, such as Trichoplusia ni Hi5

Depositor

Ning Zheng

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FBXO22 (FBXO22 Human)

Tags

GST

Expression

Insect

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-TIR1-HA

Plasmid

#172610

Purpose

Lentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

TIR1

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

Tet/ON Minimal CMV promoter

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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