We narrowed to 7,584 results for: Ski;

Showing: 6121 - 6140 of 7584 results

Results per page:

Sort results by:

pQCXIZ GFP PODXL 4K>R

Plasmid

#202420

Purpose

Expression of ubiquitination-deficient GFP-tagged PODXL (4 cytoplasmic lysines mutated to arginine)

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Mutation

K431/525/526/547R

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

Plasmid

#139097

Purpose

Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

KRAB-dxCas9-NG

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPK1520-HEK3-CTTins-ng

Plasmid

#180019

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of HEK3 CTT insertion in PE3 approach

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing ngRNA for HEK3-CTTins (EPHA8 Human)

Use

CRISPR

Expression

Mammalian

Promoter

Human U7

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
BPK1520-LRRK2-G2019S-ng

Plasmid

#180436

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of LRRK2-G2019S mutation in PE3 approach

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-pegRNA-SNCA-A53T

Plasmid

#181738

Purpose

Transiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for SNCA-A53T (SNCA Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPK1520-SNCA-A53T-ng

Plasmid

#181739

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approach

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing ngRNA for SNCA-A53T (SNCA Human)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Fgf4enhLV (FpG12)

Plasmid

#69444

Purpose

positive control plasmid for lentiviral reporter expression in ESCs

Depositor

Lisa Dailey

Article

Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

Inserts

minimal FGF4 promoter
EGFP
Ubiquitin promoter
Hygromycin resistance
FGF4 enhancer (Fgf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mCerulean-PCNA-PuroR

Plasmid

#215063

Purpose

Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Retroviral

Tags

mCerulean

Expression

Mammalian

Promoter

LTR

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX RASGAP(N)-SH2

Plasmid

#46432

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Gap/RASGAP(N) (RASA1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 168-283

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

Plasmid

#106183

Purpose

Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV1

Insert

SF-Venus-iGluSnFR.A184S

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

Promoter

hSynapsin

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.ZAC.GFAP.iAChSnFR

Plasmid

#137962

Purpose

Expresses iAChSnFR under GFAP promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Insert

iAChSnFR

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_PCCB_WT_V5

Plasmid

#82938

Purpose

Gateway Donor vector containing PCCB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PCCB (PCCB Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIBN-tagBFP-hTRF1

Plasmid

#103801

Purpose

BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-hTRF1 (CIB1, TERF1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

Plasmid

#169336

Purpose

Expression of the LT-Fucci(SA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SA) (GMNN, CDT1 Human)

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX SHP-2(C)-SH2

Plasmid

#46497

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(C) (PTPN11 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 101-222

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
mKO2-CDK4

Plasmid

#215065

Purpose

Expresses mKO2-CDK4 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Tags

mKO2

Expression

Mammalian

Promoter

LTR

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SMAD4_p.P356R

Plasmid

#82769

Purpose

Gateway Donor vector containing SMAD4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SMAD4 (SMAD4 Human)

Use

Gateway entry vector

Mutation

P356R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-pegRNA-SNCA-A30P

Plasmid

#180016

Purpose

Transiently expressing a pegRNA to introduce SNCA-A30P mutation in human cells

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for SNCA-A30P (SNCA Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPK1520-SNCA-A30P-ng

Plasmid

#180018

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A30P mutation in PE3 approach

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing ngRNA for SNCA-A30P (SNCA Human)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

Plasmid

#194190

Purpose

Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

5 mutated KLF binding sites (positions 449,548,73…

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge