We narrowed to 6,763 results for: poly
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Plasmid#186146PurposeFor expresseion of recombinant FAK using baccolovirus in SF9 cellsDepositorInsertPTK2 (PTK2 Mouse)
TagsHis6ExpressionInsectMutationC-terminal linker swapped with Pyk2 linkerPromoterpolyhedrin promoterAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSN526
Plasmid#184827PurposeExpresses human KIF5A(∆exon27) in insect cellsDepositorInsertKIF5A (∆exon27) (KIF5A Human)
TagsmScarlet-2xStrepIIExpressionInsectMutation∆exon27 form of human KIF5APromoterpolyhedrinAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
OTUB1/pRetroX-IRES-DsRed#10
Plasmid#180279PurposePlasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).DepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
IBMc397
Plasmid#161955PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHHDepositorInsertpSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
ExpressionBacterialPromoterPBADAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis
Plasmid#175788PurposePlasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184QPromoterT7Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22YU3-2xStrep
Plasmid#170315PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag IIDepositorInsertQ22YU3 (TTHERM_00122270 Tetrahymena thermophila)
TagsStrep-Tag IIExpressionInsectPromoterPolyhedrinAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP–acVHH–PB
Plasmid#169685PurposeAn anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody
Tagsmodified STIM1-PB tagExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22YU3 del C3-2xStrep
Plasmid#170316PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.DepositorInsertQ22YU3 C-terminal truncation (Delta-C3)
TagsStrep-Tag IIExpressionInsectMutationDeletion of C-terminal C3-fingerPromoterpolyhedrinAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJet-CMV-CD5cmyc2-bglpA
Plasmid#110645PurposeExpression plasmid for c-Myc-tag embedded into human CD52 with short polyADepositorInsertCMV-CDcmyc2-bglpA
ExpressionMammalianPromoterCMVAvailable SinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIMCyp (owl monkey)
Plasmid#79035PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5Cyp (owl monkey)
TagsOneSTrEP-FLAGExpressionInsectPromoterpolyhedrinAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGS-3R112C/T180C
Plasmid#85095PurposeHost vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C)DepositorInsertDNA polymerase sliding clamp 3
TagsHis6 tagExpressionBacterialMutationchanged Arginine 112 to Cysteine and Threonine 18…PromoterT7 promoterAvailable SinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-WTSI
Plasmid#80486PurposepiggyBac transposon for dox-inducible expression of the WTSI polycistronic reprogramming cassette (with mCherry)DepositorInsertWTSI
UsePiggybac transposonExpressionMammalianPromotertetOAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
JARID1BA-c539
Plasmid#79643PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertJARID1B (KDM5B Human)
UseBaculovirus expressionTags6x His and TEV cleavage sitePromoterpolyhedrinAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmUBB(SnaBI)
Plasmid#69558PurposeIn vitro transcription/translation (T7)DepositorInsertUBB (Ubb Mouse)
ExpressionBacterialMutationThe two last codons (TATTAA; encoding the last ty…PromoterT7Available SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmUBB(Y305P)
Plasmid#69557PurposeBacterial expression; in vitro transcription/translation (T7)DepositorInsertUBB (Ubb Mouse)
ExpressionBacterialMutationCodon TAT encoding tyrosine 305 was changed to CC…PromoterT7Available SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 G108C/P186C
Plasmid#66174PurposeExpresses the G108C/P186C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
TagsHis6 tagExpressionBacterialMutationG108C, P186CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR 7SK R4-R3
Plasmid#62109PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorExpressionMammalianAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR 7SK L5-L2
Plasmid#62108PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorExpressionMammalianAvailable SinceFeb. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR 7SK L5-L4
Plasmid#62107PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorExpressionMammalianAvailable SinceFeb. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR 7SK L1-R5
Plasmid#62106PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorExpressionMammalianAvailable SinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only