We narrowed to 9,894 results for: UTY

1. pCRISPaint-mScarlet-F-gB-PuroR [M1G]

   Plasmid

   #171812

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-gB-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPaint-mScarlet-F-mgp100-PuroR [M1G]

   Plasmid

   #171811

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-mgp100-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPaint-mScarlet-F-hgp100-PuroR [M1G]

   Plasmid

   #171810

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-hgp100-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPaint-mNeon-F-Ova-BlastR [M1G]

   Plasmid

   #171808

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-F-Ova-T2A-BlastR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPaint-mNeon-F-gB-BlastR [M1G]

   Plasmid

   #171807

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.M1G

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-F-gB-T2A-BlastR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRISPaint-mNeon-F-hgp100-BlastR [M1G]

   Plasmid

   #171806

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and blasticidin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-F-hgp100-T2A-BlastR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCRISPaint-mNeon-F-Ova-PuroR [M1G]

   Plasmid

   #171804

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-Ova-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. t1-405(151-154AAAA)

   Plasmid

   #166131

   Purpose

   Expression of human talin-1 head (aa1-405) in E. coli. Four substitutions in the F1-loop (aa151-154 all mutated to Al). N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site.

   Depositor

   Vesa Hytönen

   Article

   Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

   Insert

   T1head1-405(151-154AAAA) (TLN1 Human)
   

   Use

   Tags

   His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

   Expression

   Bacterial

   Mutation

   aa1-405 only. Contains 4 amino acid substitutions…

   Promoter

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Cstem(7A)-Tac(5A)

   Plasmid

   #162499

   Purpose

   Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a with mutations is inserted between GFP and Tac(5A).

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   CD8a stem region with mutations and Tac full length with mutated stem region (IL2RA, CD8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The five theronine and two serine residues are mu…

   Promoter

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)

    Plasmid

    #156413

    Purpose

    kdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sites

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4B-WT (KRAS Human)
    

    Use

    Zebrafish transgenesis

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    kdrl

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)

    Plasmid

    #156404

    Purpose

    Gateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    mTagBFP2

    Expression

    Mutation

    mutant KRAS4A-G12D

    Promoter

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. DRH015_ AAV-hSyn-WT-THR

    Plasmid

    #225088

    Purpose

    Expression of wild-type thyroid receptor beta (THRB, human) with an HA tag in neurons driven by human synapsin promoter.

    Depositor

    Bernardo Sabatini

    Article

    Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

    Insert

    WT-THRB (THRB Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Myc-gamma2/deltaILTM4

    Plasmid

    #119735

    Purpose

    GABAA receptor expression (chimeric rat rat gamma 2 subunit whose large intracellular loop, TM4 and C-term have been replaced with the equivalent domains of the rat delta subunit) C-myc near N-term

    Depositor

    Angel de Blas

    Article

    Wu et al J Biol Chem. 2012 Aug 10;287(33):27417-3

    Insert

    GABRG2/GABRD (Gabrg2, Gabrd Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. DRH016_ AAV-hSyn-DN-THR

    Plasmid

    #225089

    Purpose

    Expression of dominant-negative thyroid receptor beta (THRB, human) with an HA tag in neurons driven by human synapsin promoter.

    Depositor

    Bernardo Sabatini

    Article

    Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

    Insert

    DN-THRB (THRB Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Go1-CASE

    Plasmid

    #168123

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at L91/G92 within GNAO1

    Promoter

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Gs(short)-CASE

    Plasmid

    #168124

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG1

    Expression

    Mammalian

    Mutation

    NLuc is inserted at N136/V137 within GNAS

    Promoter

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. hNTa2-qgRNA-pYJA5

    Plasmid

    #217780

    Purpose

    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6, mouse U6, human H1, human 7SK

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. AIMTOR A757

    Plasmid

    #140829

    Purpose

    AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757

    Depositor

    Anne Bonnieu

    Article

    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

    Insert

    AIMTOR A757 (ULK1 Human)
    

    Use

    Tags

    NES Nuclear export signal

    Expression

    Mammalian

    Mutation

    The insert comprises only a small sequence of hum…

    Promoter

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-CD74-ROS1 nonmutant

    Plasmid

    #183813

    Purpose

    Express CD74-ROS1 fusion (C6;R34) in mammalian cells

    Depositor

    Aaron Hata

    Article

    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

    Inserts

    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of CD74 exon 1-6 and ROS1 exon…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-CD74-ROS1 G2032R

    Plasmid

    #183814

    Purpose

    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

    Inserts

    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    

    Use

    Retroviral

    Tags

    The fusion protein of CD74 exon 1-6 and ROS1 exon…

    Expression

    Mammalian

    Mutation

    G2032R

    Promoter

    CMV

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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