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MT363

Plasmid

#65858

Purpose

The gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

nahG

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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WT GLUT1-GFP

Plasmid

#200093

Purpose

WT human GLUT1 with a C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-TPD54

Plasmid

#172449

Purpose

Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

Insert

TPD54 (TPD52L2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

Plasmid

#192617

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAG654 pFAST

Plasmid

#172861

Purpose

Expresses pFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-TPD54

Plasmid

#172451

Purpose

Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

Insert

TPD54 (TPD52L2 Human)

Tags

mCherry-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209657

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

CAG

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209651

Purpose

Expression of iATPSnFR2, high affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Mutation

S29W.A95K

Promoter

CAG

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8s

Plasmid

#192616

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8s.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RIboL1-jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

Plasmid

#196192

Purpose

human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4

Use

Znf donor

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pArcCreER

Plasmid

#46389

Depositor

Liqun Luo

Article

Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

Inserts

Arc (Arc Mouse)
CreERT2

Use

Mouse Targeting

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pSEM319 - array co-injection fragment mosTI unc-119

Plasmid

#159823

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI unc-119

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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phage ubc nls ha 2xmcp HALO

Plasmid

#64540

Purpose

Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

MCP

Use

Lentiviral

Tags

HA, HALO, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pPI226

Plasmid

#113228

Purpose

safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-mCherry

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

LifeAct

Use

Safe haven knock-in plasmid for the act5 locus, l…

Tags

mCherry

Promoter

act5

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Thy1 promoter construct

Plasmid

#20736

Depositor

Joshua Sanes

Article

Feng et al Neuron. 2000 Oct . 28(1):41-51.

Insert

Thy1 promoter (Thy1 Mouse)

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209656

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Mutation

A95K

Promoter

CAG

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95A.A119L.mRuby3

Plasmid

#244913

Purpose

iATPSnFR2.A95A.A119L variant with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.mRuby3

Use

AAV

Mutation

A95A.A119L

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pJW1512

Plasmid

#62366

Purpose

N-terminal 3X FLAG fused to wt BirA - mCherry - sec61ß

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pTRE2

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pPotef-cdc3CB16-NatR

Plasmid

#86465

Purpose

Expresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.

Depositor

Michael Feldbrügge

Article

Baumann et al EMBO Rep. 2014 Jan;15(1):94-102. doi: 10

Insert

Cdc3

Use

Ustilago maydis expression

Tags

mCherry

Expression

Bacterial

Promoter

Potef

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-wt-TTF-1

Plasmid

#49989

Purpose

For transient transfection to express human wild type TTF-1 protein

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

TTF-1 (NKX2-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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