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pTRIP-SFFV-mtagBFP-2A

Plasmid

#102585

Purpose

Control lentivector. Coexpression of mTagBFP and gene of interest from SFFV promoter

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-RaichuEV-Rac1HRasCT

Plasmid

#214818

Purpose

Encoding a Förster resonance energy transfer (FRET) biosensor for Rac1 activity with cyan and yellow fluorescent protein.

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

RaichuEV-Rac1HRasCT

Tags

HRasCAAX

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6M-Pak1 L107F

Plasmid

#12212

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Constitutively active Pak1: L107F

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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HA-GbL

Plasmid

#1865

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

GbL (MLST8 Human)

Tags

HA

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-XRCC4

Plasmid

#46959

Purpose

Plasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

XRCC4 (XRCC4 Human)

Tags

GFP-2xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRoquin2_AE

Plasmid

#148614

Purpose

Mammalian Expression of HsRoquin2

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin2 (RC3H2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-PIS

Plasmid

#119078

Purpose

Visualize localization of ER enzyme Phosphatidylinositol Synthase (PIS)

Depositor

Gia Voeltz

Article

English et al Nat Cell Biol. 2013 Feb;15(2):169-78. do

Insert

PIS (CDIPT Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-flag-GFP-JAM-B

Plasmid

#205191

Purpose

This vector encodes of a synCAM (JAM-B) and GFP

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM JAM-B and GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-TMCC2

Plasmid

#121047

Purpose

Expresses human TMCC2 in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Insert

Trans membrane and coiled coil domain containing protein 2 (TMCC2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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hNCAM2

Plasmid

#84473

Purpose

expression of hNCAM2 in mammalian cells

Depositor

Vladimir Sytnyk

Article

Leshchyns'ka et al Nat Commun. 2015 Nov 27;6:8836. doi: 10.

Insert

NCAM2 (NCAM2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-myrlysin/BORCS5

Plasmid

#194332

Purpose

Expresses myrlysin in mammalian cells

Depositor

Juan Bonifacino

Article

Pu et al Dev Cell. 2015 Apr 20;33(2):176-88. doi:

Insert

BORCS5 (BORCS5 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLPCX-PECAMTL

Plasmid

#45851

Depositor

Martin Schwartz

Article

Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

Insert

PECAM1 (PECAM1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter pGL3Basic

Plasmid

#32727

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Luciferase reporter gene

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-BFLF1

Plasmid

#162657

Purpose

Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pTC217

Plasmid

#70018

Purpose

Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (Cas9/sgRNA) + Donor + GVR

Use

CRISPR

Expression

Plant

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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mcherry-Myo 9b

Plasmid

#134918

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.

Depositor

Martin Bähler

Article

Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

Insert

Myo 9b (rat) with 3' UTR (Myo9b Rat)

Tags

mcherry

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV2-hNCOA6:V5

Plasmid

#63585

Purpose

A subunit of the Trithorax-related (Trr) methyltransferase complex.

Depositor

Kenneth Irvine

Article

Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

Insert

NCOA6 (NCOA6 Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA_DENV2-NS2B3_V5

Plasmid

#115906

Purpose

Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tag

Depositor

Alan Rothman

Article

Udawatte et al Front Cell Infect Microbiol. 2022 Oct 14

Insert

DENV2 NS2B3 protease

Tags

V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR5'-attP (pCM254)

Plasmid

#68311

Purpose

Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.

Depositor

Christian Mosimann, Leonard Zon

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Insert

attP

Use

Gateway entry vector

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pAR-Ec611

Plasmid

#130872

Purpose

A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (I777K, L900S) for OrthoRep

Depositor

Chang Liu

Article

Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

Insert

TP-DNAP1

Expression

Yeast

Mutation

I777K, L900S

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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