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Showing: 601 - 620 of 66158 results

  5. pCRIS-PITChv2-dTAG-BSD (BRD4)

    Plasmid
    #91795
    Purpose
    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.
    Depositor
    James Bradner, Behnam Nabet
    Publication
    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP_F36V-2xHA-P2A-BSD
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Phenylalanine 36 to valine in FKBP12
    Promoter
    Promotorless
    Available since
    March 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91795-plasmid-map-sequence-id-197291

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  10. pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro

    Plasmid
    #141385
    Purpose
    Lentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-E (E SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 8, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141385-plasmid-map-sequence-id-288068

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  11. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS, No promoter, and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60224-plasmid-map-sequence-id-418949

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  12. CMV-B-GECO1

    Plasmid
    #32448
    Purpose
    Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Publication
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-GECO1.0
    Expression
    Mammalian
    Mutation
    GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…
    Promoter
    CMV
    Available since
    Sept. 30, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    32448-plasmid-map-sequence-id-122739

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  14. CMV-mito-GEM-GECO1

    Plasmid
    #32461
    Purpose
    Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Publication
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GEM-GECO
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    CMV
    Available since
    Dec. 5, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    32461-plasmid-map-sequence-id-123705

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Showing: 601 - 620 of 66158 results