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pRSV-CaMKIV(1-313)

Plasmid

#45063

Purpose

Expression of constit active CaMKIV

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Cam Kinase IV(1-313) (Camk4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

truncated at amino acid 313*

Promoter

RSV

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET his-mRFP

Plasmid

#113553

Purpose

For bacterial expression and purification of mRFP

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

his-mRFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRSITEP-puro-shWRN2

Plasmid

#125785

Purpose

DOX-inducible expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-GFP

Plasmid

#166836

Purpose

Expresses Vac8-GFP in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCSDest-MYC-TMPRSS2

Plasmid

#154964

Purpose

TMPRSS2 with N-terminal MYC

Depositor

Utpal Pajvani

Article

Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

Insert

MYC-TMPRSS2 (TMPRSS2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRSFDuet-1_VNp-FGF21

Plasmid

#182407

Purpose

Bacterial expression of Vesicle Nucleating peptide-fgf21 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-fgf21 (FGF21 Human)

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRS416gT-GalL-Cas9

Plasmid

#79903

Purpose

pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression

Depositor

Ronald Davis

Article

Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw

Inserts

Cas9
guide RNA (gRNA)
Tetracycline Repressor

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

GPM1 promoter, GalL, and RPR1 promoter with TetO …

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pRS416-Aga1p-FAPB2.3.6L1TAG

Plasmid

#158148

Purpose

Yeast display reporter for non-display yeast strains (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p-FAPB2.3.6

Use

Tags

Expression

Yeast

Mutation

Cloned in a TAG codon at the first position of th…

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETa-mEos3.2-A69T

Plasmid

#98571

Purpose

photoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mEos3.2-A69T

Use

Tags

7xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRS316CUP RNQ CFP

Plasmid

#1171

Depositor

Susan Lindquist

Article

Sondheimer et al Mol Cell 2000 Jan;5(1):163-72.

Insert

RNQ1 (RNQ1 Budding Yeast)

Use

Tags

CFP

Expression

Yeast

Mutation

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pAS_4xEcoLeuT(CUA)_EF1_AnapRS

Plasmid

#174894

Purpose

AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AnapRS

Use

Tags

Expression

Mammalian

Mutation

L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRS426 TEF mCherry

Plasmid

#129433

Purpose

express mcherry

Depositor

Oskar Laur

Insert

mcherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-Tmprss6(Mask)

Plasmid

#18790

Depositor

Bruce Beutler

Article

Du et al Science. 2008 May 23. 320(5879):1088-92.

Insert

Type II transmembrane serine protease 6 (Tmprss6 Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 567-811 and replaced with 28 …

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pBJ1807 pRS415-COF1RFP

Plasmid

#37102

Depositor

John Cooper

Article

Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

Insert

COF1-RFP

Use

Tags

RFP

Expression

Yeast

Mutation

Single RFP internal insertion at Cof147T48D with …

Promoter

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-baPrs-hdNadV

Plasmid

#91950

Purpose

pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

Depositor

George Cătălin Marinescu

Article

Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

Inserts

Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases

Use

Tags

Expression

Bacterial

Mutation

changed Leucine 135 to Isoleucine

Promoter

T7

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRS305-mAID-LaM4

Plasmid

#198414

Purpose

Express mAID-Nb(LaM4) under the control of ADH1 promoter in budding yeast

Depositor

Takumi Kamura

Article

Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

Insert

mAID-Nb (LaM4)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1 promoter

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRS305-mAID-LaM8

Plasmid

#198415

Purpose

Expresesses mAID-Nb(LaM8) under the control of ADH1 promoter in budding yeast

Depositor

Takumi Kamura

Article

Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

Insert

mAID-Nb (LaM8)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1 promoter

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2-Cterminal6xHis

Plasmid

#60386

Purpose

Expression of yeast beta tubulin (TUB2)-Cterminal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

6xHIS

Expression

Yeast

Mutation

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pBJ1808 pRS416-COF1RFP

Plasmid

#37103

Depositor

John Cooper

Article

Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

Insert

COF1-RFP

Use

Tags

RFP

Expression

Yeast

Mutation

Single RFP internal insertion at Cof147T48D with …

Promoter

Available since

June 19, 2012

Availability

Academic Institutions and Nonprofits only
pLEX307-TMPRSS4-G418

Plasmid

#158474

Purpose

Constitutive lentiviral expression of human TMPRSS4.

Depositor

Sho Iketani, Alejandro Chavez

Insert

TMPRSS4 (TMPRSS4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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