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FLAG-MKK6 (S207E,T211E)-pcw107

Plasmid

#64625

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K6 (MAP2K6 Human)

Use

Lentiviral

Tags

FLAG

Mutation

S207E,T211E

Promoter

PGK

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223_MAPK8_WT

Plasmid

#82229

Purpose

Gateway Donor vector containing MAPK8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK8 (MAPK8 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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MAP2K4 gRNA (BRDN0001145410)

Plasmid

#76651

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K4 (MAP2K4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K4 gRNA (BRDN0001148854)

Plasmid

#76652

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K4 (MAP2K4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pT22Ubm5En2SRE4PCh(#302)

Plasmid

#184089

Purpose

cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-chkEn2(SR)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Mutation

chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7B2Jnk1a1(APF)

Plasmid

#19730

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

MKK7B2Jnk1a1(APF) (MAPK8, Map2k7 Mouse, Human)

Tags

Flag

Expression

Mammalian

Mutation

replaced dual phosporylation motif Thr(1959)-Pro-…

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1 MKK4(EE)-MKK7a1(EE)

Plasmid

#47580

Purpose

Bacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Inserts

MKK4-EE (MAP2K4 Human)
MKK7a1-EE (Map2k7 Mouse)

Expression

Bacterial

Mutation

Lacks the first 36 residues of the RefSeq protein…

Available Since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only
pDmelOR-mRho.V5.mER.hOr56a

Plasmid

#126479

Purpose

Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Inserts

Or56a (Or56a Fly)
Orco (Orco Fly)

Tags

H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

Expression

Mammalian

Mutation

codon optimization for H. sapiens and codon optim…

Promoter

CMV

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAPK12

Plasmid

#20592

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAPK12 (MAPK12 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP2K7

Plasmid

#23798

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP2K7 (MAP2K7 Human)

Use

Gateway donor vector

Mutation

F425L

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK12

Plasmid

#23719

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK12 (MAPK12 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag MAP2K7

Plasmid

#20516

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP2K7 (MAP2K7 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223_MAP2K4_WT

Plasmid

#82142

Purpose

Gateway Donor vector containing MAP2K4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K4 (MAP2K4 Human)

Use

Gateway entry vector

Mutation

P27R; p.8delG

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK8

Plasmid

#23420

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK8 (MAPK8 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAPK10

Plasmid

#23649

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK10 (MAPK10 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP2K6

Plasmid

#23783

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP2K6 (MAP2K6 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pHAGE-MAP2K4-G294E

Plasmid

#116430

Purpose

Lentiviral expression of MAP2K4 G294E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP2K4 (MAP2K4 Human)

Use

Lentiviral

Mutation

G294E

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K4_p.S184L

Plasmid

#81681

Purpose

Gateway Donor vector containing MAP2K4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K4 (MAP2K4 Human)

Use

Gateway entry vector

Mutation

S184L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAP2K6

Plasmid

#20515

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP2K6 (MAP2K6 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDmelOR-mRho.V5.mER.hOr47a

Plasmid

#126478

Purpose

Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Inserts

Or47a (Or47a Fly)
Orco (Orco Fly)

Tags

H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

Expression

Mammalian

Mutation

codon optimization for H. sapiens and codon optim…

Promoter

CMV

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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