We narrowed to 10,263 results for: ADA

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Flag-neo-PP1-Alpha-H248K

Plasmid

#171268

Purpose

Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant that loses enzymatic activity

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant (PPP1CA Human)

Tags

Flag

Expression

Mammalian

Mutation

PPP1CA-His248Lys (H248K)

Promoter

CMV

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-mScarlet-I-CD4-bla

Plasmid

#179442

Purpose

Donor vector to knock in mScarlet-I C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

mScarletI

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

Plasmid

#92140

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

AID[71-114]-eGFP

Use

Mouse Targeting

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

Plasmid

#178528

Purpose

Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

rtTA, SspB-mScarlet-I-MYPT (1-169aa)

Expression

Mammalian

Promoter

CAG, Tet responsive element

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-ABE7

Plasmid

#193644

Purpose

Expression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA-TadA*7.10, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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mP58.dJ1-pCDNA3

Plasmid

#21884

Depositor

David Ron

Article

Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

Insert

P58 (Dnajc3 Mouse)

Expression

Mammalian

Mutation

DnaJ domain deleted from C-term

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pLX304 3xFlag-eRF1 G183/184A_IRES-GFP

Plasmid

#198394

Purpose

eRF1 (AAQ mutant) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

eRF1 (ETF1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

mutated GGQ motif (G183/184A)

Promoter

CMV

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin1.OxLight-ctr

Plasmid

#169793

Purpose

Control non-binding fluorescent reporter for orexin neuropeptides

Depositor

Tommaso Patriarchi

Article

Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

Insert

OxLight-ctr

Use

AAV

Mutation

E54K; T111A

Promoter

human Synapsin-1

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-EMTB-TurboID-V5

Plasmid

#190736

Purpose

Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Article

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Insert

ensconsin (MAP7 Human)

Tags

Myc, TurboID, and V5

Expression

Mammalian

Mutation

Microtubule binding-domain of ensconsin (amino ac…

Promoter

CMV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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mCh-BICD2*-strep

Plasmid

#120168

Purpose

Expresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

BICD cargo adaptor 2 (Bicd2 Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated BICD2: 15-595 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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AAV2_CAG_oROS-HT_WPRE

Plasmid

#216414

Purpose

Encodes the genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR S286/287/291A

Plasmid

#159160

Purpose

Expresses DEPTOR S286/287/291A mutant in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Mutation

changed serine 286,287 and 291 to alanine

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR S286/287/291D

Plasmid

#159161

Purpose

Expresses DEPTOR S286/287/291D mutant in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Mutation

changed serine 286,287 and 291 to aspartic acid

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-ABE8e-V106W

Plasmid

#193646

Purpose

Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e-V106W, EGFP

Expression

Mammalian

Mutation

TadA*8e-V106W

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast HA-POLE2

Plasmid

#160809

Purpose

Express HA-tagged POLE2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

HA-POLE2 (POLE2 Human)

Use

Retroviral

Tags

HA

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-KLF4

Plasmid

#130694

Purpose

Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human KLF4 (KLF4 Human)

Use

Lentiviral

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1a-hMLH1dn (og codon)

Plasmid

#174823

Purpose

Mammalian expression of human MLH1dn (original codon usage)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (original codon usage) (MLH1 Human)

Expression

Mammalian

Mutation

deletion of residues 754-756

Promoter

EF1a

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSD138A

Plasmid

#218130

Purpose

Hybrid circuit displaying hsCRBN bait (P22 anchor) and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN (CRBN Human)
luxAB

Tags

scP22

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-LAMP1

Plasmid

#222318

Purpose

Synchronize the trafficking of LAMP1 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1 fused to SBP-mCherry (LAMP1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-DCX-TurboID-V5-puro

Plasmid

#190742

Purpose

A lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Article

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Insert

DCX-TurboID-V5 (DCX DCX is human doublecortin; TurboID is engineered BirA from E.coli, Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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