We narrowed to 16,915 results for: EMB

1. EZ-L498

   Plasmid

   #131771

   Purpose

   PPGK1_FUSN_Citrine _PixE_TCYC1

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   PPGK1_FUSN_Citrine _PixE_TCYC1
   

   Use

   Integration into his3 locus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMaCTag-09

   Plasmid

   #119988

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with mVenus

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mVenus
   

   Use

   Pcr template

   Tags

   mVenus

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. TAL2452

   Plasmid

   #36841

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   RUNX1-TALEN_Left (Runx1 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pMTB-Multibow-mR

   Plasmid

   #60990

   Purpose

   Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound dTomato

   Depositor

   Sean Megason

   Article

   Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

   Insert

   dTomato
   

   Use

   Zebrafish

   Tags

   membrane tag

   Expression

   Mutation

   Promoter

   Bactin2

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCMB-TLEr

   Plasmid

   #61188

   Purpose

   Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   MtRAB7A2
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   MtBCP1

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-AcrIIA4

   Plasmid

   #174034

   Purpose

   In vitro transcription of anti-CRISPR AcrIIA4 mRNA

   Depositor

   James Gagnon

   Article

   Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

   Insert

   AcrIIA4
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-AcrIIA2

   Plasmid

   #174033

   Purpose

   In vitro transcription of anti-CRISPR AcrIIA2 mRNA

   Depositor

   James Gagnon

   Article

   Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

   Insert

   AcrIIA2
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWZ414-F55

   Plasmid

   #23079

   Depositor

   Sharon Dent

   Article

   Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

   Insert

   yeast Histone H3-2 and Histone H4-2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Histone H3 changed Lysine 9 to Arginine, H3 K9R …

   Promoter

   Available since

   March 3, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pMaCTag-29

   Plasmid

   #120008

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with Strep-II

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Strep-II
   

   Use

   Pcr template

   Tags

   Strep-II

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHJ386

    Plasmid

    #20679

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Moesin (MSN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Lys 253, 254, 262, 263 to Asn; Changed Th…

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pMMBAD16

    Plasmid

    #20176

    Depositor

    Hideo Iwai

    Article

    Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

    Insert

    src homology domain 3 (CRK Human)
    

    Use

    Tags

    NpuDnaEc16

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcryBFP_Umyo18bGFP

    Plasmid

    #172527

    Purpose

    alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.

    Depositor

    Joachim Berger

    Article

    Berger et al Hum Mol Genet. 2017 Mar 15;26(6):1146-11

    Insert

    myo18b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA1

    Plasmid

    #46789

    Purpose

    To express Fun30 in trans in budding yeast

    Depositor

    Patrick Varga-Weisz

    Article

    Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

    Insert

    FUN30 (FUN30 Budding Yeast)
    

    Use

    Tags

    V5

    Expression

    Yeast

    Mutation

    Promoter

    PGAL1

    Available since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pc3XB-ZF70

    Plasmid

    #13193

    Depositor

    Daniel Voytas

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF70
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. Venus-7aa-MAO-pEGFP-C3

    Plasmid

    #166765

    Purpose

    To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

    Depositor

    David Andrews

    Insert

    MAO tail anchor (MAOA Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-kanMX6-P41nmt1-mYFP

    Plasmid

    #105141

    Purpose

    Yeast gene targeting

    Depositor

    Thomas Pollard, Jian-Qiu Wu

    Article

    Wu et al Dev Cell. 2003 Nov;5(5):723-34.

    Insert

    kanMX6-P41nmt1-mYFP
    

    Use

    Tags

    41nmt1-mYFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    41nmt1

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCherry-C1-DH

    Plasmid

    #67898

    Purpose

    Expresses a fusion of mCherry with the DH domain (155-347) of p63RhoGEF in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    mCherry-DH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOGG202

    Plasmid

    #115503

    Purpose

    Clone of a level 1 unit which encodes IPTG-inducible sfGFP

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    pL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. C512-E04: attB5r-mCherry-nostop-attB1r

    Plasmid

    #162941

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal mCherry fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    monomeric Cherry fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. UBQ10:sXVE:ER-GFP1–10

    Plasmid

    #125664

    Purpose

    Binary vector for the inducible expression of ER-targeted GFP1–10 fragment that can be used to detect a protein tagged with GFP11

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Methods Mol Biol. 2021;2200:323-336. doi

    Insert

    ER-GFP1–10
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    UBQ10:sXVE:

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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