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pTight-9-124

Plasmid

#31874

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

miR-9/9* and miR-124 (Mir124a-2, Mir9-3 Mouse)

Use

Lentiviral

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5B-CMV-GFP

Plasmid

#83257

Purpose

Adenoviral expression of STAT5B with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5b (Stat5b Mouse)
eGFP

Use

Adenoviral

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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E228Q Vps4A-HA

Plasmid

#200087

Purpose

3x HA-VPS4A with E228Q dominant negative mutation in Dox-inducible pInducer20 vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

VPS4A (VPS4A Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

E228Q

Promoter

CMV

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-FLEX-eNpHR-EYFP

Plasmid

#89876

Purpose

To generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

eNpHR=EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Promoter

TetO

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP4

Plasmid

#42313

Depositor

Thierry Galli

Article

Mallard et al J Cell Biol. 2002 Feb 18;156(4):653-64.

Insert

VAMP 4 (VAMP4 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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AAVS1-PuroR Xlone eGFP

Plasmid

#179837

Purpose

Doxycycline-inducible expression of eGFP

Depositor

Xiaoping Bao

Article

Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

Insert

eGFP

Use

CRISPR, Synthetic Biology, and TALEN ; Donor plas…

Expression

Mammalian

Promoter

TRE3GS

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCHA1.1-MFSD12-V5

Plasmid

#149508

Purpose

Stable expression of human MFSD12 with recoded nucleotide sequence.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

MFSD12 (MFSD12 Human)

Use

Lentiviral

Tags

V5

Mutation

Codon Optimized Sequence

Promoter

Ubc

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDAS12395_PEAR-GFP_2.0-preedited

Plasmid

#177185

Purpose

PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDGB1a2+pNOS_Red-F_NOSt

Plasmid

#170887

Purpose

GoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Forward orientation

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

Red-F

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

p.S286Y Red mutant

Promoter

pNOS

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A2

Plasmid

#131964

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A2 (SLC22A2 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-ChRmine-mRuby2-ST

Plasmid

#227777

Purpose

Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChRmine-mRuby2-ST

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-mU6-GFPGO2-IRES-mScarletI

Plasmid

#136896

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPGO2-IRES-mScarletI

Use

Lentiviral

Tags

2xNLS on GFP, 3xNLS on mScarlet

Mutation

ATG>ACG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBa.KIF13B

Plasmid

#224413

Purpose

Mammalian expression of Kif 13b

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

Kif 13b (Kif13b Mouse)

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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CD11b promoter in pGEM3zf(-)

Plasmid

#26168

Depositor

Daniel Tenen

Article

Dziennis et al Blood. 1995 Jan 15. 85(2):319-29.

Insert

CD11b promoter (ITGAM Human)

Expression

Bacterial

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pCHW101020

Plasmid

#222454

Purpose

Level 1, Position 2 containing 35S::CUP2-p65::NOS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

35S::CUP2-p65::NOS (CUP2 )

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLHCX-gpASNase1

Plasmid

#121526

Purpose

Expresses gpASNase1 in mammalian cells.

Depositor

Lucas Sullivan, Matthew Vander Heiden

Article

Sullivan et al Nat Cell Biol. 2018 Jul;20(7):782-788. d

Insert

gpASNase1

Use

Retroviral

Tags

FLAG

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCHW101008

Plasmid

#222453

Purpose

Level 1, Position 5 containing CBS4-miniDFR::OsL5::NOS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-miniDFR::OsL5::NOS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pConRef-2H12

Plasmid

#221182

Purpose

cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

Constitutive and None (same as cdGreen)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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Circular-oc-EMCV-GFP

Plasmid

#226261

Purpose

Production of in vitro circularizable RNA via IVT

Depositor

Prashant Mali

Article

Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

Insert

EGFP

Use

In vitro transcription

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEYFP-Sec24C

Plasmid

#66608

Purpose

YFP fusion of Sec24C

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec24C (SEC24C Human)

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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