We narrowed to 23,593 results for: promoter

1. pL-CRISPR.SFFV.tRFP

   Plasmid

   #57826

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   SFFV
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB535

   Plasmid

   #74873

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   No Promoter

   Available Since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9::BFP-humanized

   Plasmid

   #44247

   Purpose

   Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 fused to BFP
   

   Use

   CRISPR and Retroviral

   Tags

   2xNLS & tagBFP

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   MSCV LTR promoter

   Available Since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_355

   Plasmid

   #187159

   Purpose

   Lentiviral expression of doxycycline inducible customizable Sp Cas9 sgRNA

   Depositor

   David Root, John Doench

   Article

   Aquilanti et al Neuro Oncol. 2023 Jul 6;25(7):1275-1285.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX307 ASC

   Plasmid

   #163977

   Purpose

   Mammalian expression vector encoding ASC (for example, to assess ASC-speck formation or inflammasome signalling assays)

   Depositor

   Daniel Bachovchin

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   ASC (PYCARD Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Accessory plasmid 1

   Plasmid

   #128569

   Purpose

   for mixing with SPECS library to produce lentivirus

   Depositor

   Timothy Lu

   Article

   Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

   Insert

   ECFP
   

   Use

   Lentiviral

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-Camk2d

   Plasmid

   #186820

   Purpose

   Fluorescent reporter for Camk2d expression

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   Camk2d promoter (Camk2d Mouse)
   

   Use

   Luciferase

   Promoter

   Camk2d promoter

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 9X NFAT-apha-MHC-Luc

   Plasmid

   #51941

   Purpose

   9 copies of NFAT sites in minimal alphaMHC-pGL-3

   Depositor

   Jeffery Molkentin

   Article

   Wilkins et al Circ Res. 2004 Jan 9;94(1):110-8. Epub 2

   Inserts

   9x NFAT binding site
   alpha-MHC
   

   Use

   Luciferase

   Tags

   luciferase

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB553

   Plasmid

   #74883

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   No Promoter

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZW12 (CK2beta)

    Plasmid

    #27088

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-UBC-ERT2-iCre-ERT2

    Plasmid

    #178060

    Purpose

    Conditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ERT2-iCre-ERT2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUCXMG

    Plasmid

    #204409

    Purpose

    Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

    Depositor

    David Ackerley

    Article

    Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-GFP-FUS-IDR

    Plasmid

    #183236

    Purpose

    Expression of eGFP fused to a intrinsically disordered regions of the FUS protein

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    EGFP
    

    Tags

    FLAG tag and Fusion to an IDR from the FUS protei…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX304-mNeonGreen

    Plasmid

    #162034

    Purpose

    Tagged vector control

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Neon Green

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. P25-pZE-SUMO-PbTTA

    Plasmid

    #204634

    Purpose

    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

    Depositor

    Aditya Kunjapur

    Article

    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

    Insert

    His-SUMO-TEV-PbTTA
    

    Use

    Synthetic Biology

    Tags

    His Tag; SUMO Tag; TEV Protease Site

    Expression

    Bacterial

    Promoter

    PLtetO-1 promoter

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKRG3-mU6-PUb-3xFLAG-hSpCas9

    Plasmid

    #162161

    Purpose

    Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Insect

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB453

    Plasmid

    #74835

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    No Promoter

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 myc card9

    Plasmid

    #16253

    Depositor

    William Kaelin

    Article

    Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

    Insert

    card9 (CARD9 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Nov. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVH-EF1a-GFP-P2A-SOX2

    Plasmid

    #130693

    Purpose

    Lentiviral plasmids encoding human SOX2 with co-expressoin of GFP mediated by P2A.

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    human SOX2 (SOX2 Human)
    

    Use

    Lentiviral

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRE3G-Bi-gePSI-pGFAP-AcGFP

    Plasmid

    #133733

    Purpose

    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a GFAP promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    

    Use

    AAV

    Tags

    murine ornithine decarboxylase - degron (ODC36)

    Expression

    Mammalian

    Promoter

    pGFAP and pTRE3G-Bi

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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