162,717 results

1. pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

   Plasmid

   #198035

   Purpose

   Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   SINTBAD (TBKBP1 Human)
   

   Use

   Tags

   EGFP and GST-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. BII-ChPtW-iRFP670

   Plasmid

   #133397

   Purpose

   Piggybac vector for constitutive expression of iRFP670-NLS

   Depositor

   Blair Madison

   Insert

   iRFP-670-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC28-SENP2

   Plasmid

   #196355

   Purpose

   protein expression of the His-tagged catalytic domain

   Depositor

   Stefan Knapp

   Insert

   SENP2 catalytic domain
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   contains only D364-L589

   Promoter

   T7

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX307-CTSB-blast

   Plasmid

   #158464

   Purpose

   Constitutive lentiviral expression of human CTSB.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   CTSB (CTSB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-enCas12a-A3A-Y130F

   Plasmid

   #193652

   Purpose

   Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLb-enCas12a-hA3A-Y130F, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F

   Promoter

   CMV

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dCas12a-A3A-Y132D

   Plasmid

   #193641

   Purpose

   Expression of base editor dCas12a-A3A-Y132D in mammalian cells for base editing

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3A-Y132D
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A-Y132D

   Promoter

   CMV

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21-pelB-LaM-6

   Plasmid

   #172771

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-6
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSV-PKImut-v2

   Plasmid

   #45067

   Purpose

   Expression of intactive PKI (PKA inhibitor) mutant

   Depositor

   Richard Maurer

   Insert

   cAMP-dependent protein kinase inhibitor alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Arg 20 & 21 to Glycines

   Promoter

   RSV

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGK-H2BeGFP

   Plasmid

   #21210

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   Phosphoglycerate kinase promoter (PGK1 Human)
   

   Use

   Lentiviral

   Tags

   H2B-eGFP

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-6P-1-GST-CD(Cbx7)

    Plasmid

    #82525

    Purpose

    Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 chromodomain only; amino acids 1–62.

    Depositor

    Xiaojun Ren

    Insert

    Chromobox Homolog 7 (CBX7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    CD(Cbx7) ; amino acids 1–62

    Promoter

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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