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pX462-hPRKAA1-gRNA_A

Plasmid

#74374

Purpose

gRNA_A to knockout human AMPK alpha 1 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)

Use

CRISPR

Promoter

U6

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-hTFIIB

Plasmid

#171084

Purpose

Expresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIB

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

TF2B (GTF2B Human)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEND-227_Cacnes_mCerulean

Plasmid

#225614

Purpose

Replicative vector for blue fluorescent reporter protein mCerulean strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCerulean.

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

mCerulean

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCrysβ:ECFP-LexOP:mCherry-KrasG12V

Plasmid

#89888

Purpose

Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-KRasG12V

Use

Unspecified

Tags

mCherry

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE HA Chk2 WT

Plasmid

#41901

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

CHK2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-2strep-FBH1

Plasmid

#236464

Purpose

transient overexpression of FBH1 in mammalian cells

Depositor

Michele Pagano

Article

Jeong et al J Cell Biol. 2013 Jan 21;200(2):141-9. d

Insert

FBH1 (FBXO18 Human)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pMJ117

Plasmid

#85997

Purpose

One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr3
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified human U6

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/VEGF/IRES/EGFP

Plasmid

#206211

Purpose

An expression vector of VEGF and EGFP under control of TRE promoter

Depositor

Masamichi Kamihira

Article

Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

Insert

vascular endothelial growth factor (Vegfa Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ179

Plasmid

#85996

Purpose

One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified mouse U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-SorLA-v1

Plasmid

#154892

Purpose

Expresses SorLA fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

SORL1 (SORL1 Human)

Tags

Venus fragment 1 (v1)

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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F-IRF7

Plasmid

#169777

Purpose

Express IRF7 in mammalian cells

Depositor

Sung-Liang Yu

Article

Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

Insert

interferon regulatory factor 7 (IRF7), transcript variant a (IRF7 Human)

Tags

Flag tag

Expression

Bacterial and Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-BEACON2

Plasmid

#171698

Purpose

Expresses BEACON2 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

Insert

BEACON2

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_W98Y/W104A/Y130F

Promoter

CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hSTING (S366A)

Plasmid

#214155

Purpose

Transient expression of STING

Depositor

Nan Yan

Article

Wu et al Immunity. 2020 Jul 14;53(1):115-126.e5.

Insert

STING (S366A) (STING1 Human)

Expression

Mammalian

Mutation

S366A

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR mouse Tnf

Plasmid

#207127

Purpose

Luciferase vector containing 3'UTR for mouse Tnf

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Tumor necrosis factor (Tnf) 3'UTR (Tnf Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PGK promoter

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-recA56

Plasmid

#89962

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet and proD

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-LMO7 (mNeonGreen-eKL9h-VChR1)

Plasmid

#205097

Purpose

fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Björefeldt et al Neurophotonics. 2024 Apr;11(2):021005. d

Insert

mNeonGreen, eKaz luciferase, Volvox Channelrhodopsin 1

Use

AAV

Promoter

hSyn

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-Wild-Type

Plasmid

#235169

Purpose

Expresses Wild-Type GRP78 in mammalian cells

Depositor

Amy Shiu Lee

Article

Zhang et al J Biol Chem. 2010 May 14;285(20):15065-1

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)

Plasmid

#191650

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSCALPs-HA-NFAT1 (4-460)-GFP

Plasmid

#88879

Purpose

Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)

Use

Lentiviral

Tags

GFP fusion protein and HA tag

Expression

Mammalian

Mutation

amino acids 4-460 only; L78P & L287P *see bel…

Promoter

SFFV (spleen focus forming virus)

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-NurRE3

Plasmid

#208253

Purpose

pGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cells

Depositor

Douglas Kojetin

Article

Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

Insert

3X Nur response element LUC

Tags

No

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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